# Latest **URL:** https://help.galaxyproject.org/latest.md?no_subcategories=false [Latest](https://help.galaxyproject.org/latest.md) · [Categories](https://help.galaxyproject.org/categories.md) · [Tags](https://help.galaxyproject.org/tags.md) --- ## [How to access your Self Serve extended data storage at UseGalaxy servers!](https://help.galaxyproject.org/t/how-to-access-your-self-serve-extended-data-storage-at-usegalaxy-servers/13193)
**Author:** [@swagat23](https://help.galaxyproject.org/u/swagat23)\ **Replies:** 1\ **Last updated:** [August 12, 2024, 5:56pm UTC](https://help.galaxyproject.org/t/how-to-access-your-self-serve-extended-data-storage-at-usegalaxy-servers/13193 "2024-08-12T17:56:47Z")
I’m working on a transciptomics data which will probably exceed the given 250GB data. Is there any possible solution to this? for example increasing my storage? --- ## [Why collections?](https://help.galaxyproject.org/t/why-collections/16411)
**Author:** [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Replies:** 0\ **Last updated:** [October 21, 2025, 10:41pm UTC](https://help.galaxyproject.org/t/why-collections/16411 "2025-10-21T22:41:50Z")
Collections are one of the super-killer features of Galaxy! By Anton Nekrutenko October 21, 2025 --- ## [MultiQC Dataset selector stuck](https://help.galaxyproject.org/t/multiqc-dataset-selector-stuck/18437)
**Author:** [@aaak](https://help.galaxyproject.org/u/aaak)\ **Replies:** 2\ **Last updated:** [October 3, 2026, 7:22am UTC](https://help.galaxyproject.org/t/multiqc-dataset-selector-stuck/18437 "2026-10-03T07:22:48Z")
Hello, I am experiencing an issue with the dataset selector in Galaxy Europe while configuring MultiQC. I have approximately 130 STAR-related datasets in my history. When I open the STAR log output selector in MultiQC,… --- ## [miRge3 reference library not available in the server](https://help.galaxyproject.org/t/mirge3-reference-library-not-available-in-the-server/18424)
**Author:** [@nive3437](https://help.galaxyproject.org/u/nive3437)\ **Replies:** 6\ **Last updated:** [October 3, 2026, 7:08am UTC](https://help.galaxyproject.org/t/mirge3-reference-library-not-available-in-the-server/18424 "2026-10-03T07:08:42Z")
Admins, please fix the following issue I’m trying to use the miRge3 pipeline for miRNA seq analyses (done using the MGI platform), the miRge3 human library is not available (nor can it be manually uploaded) --- ## [Metatranscriptomic analysis fails to determine pathwasy with HUMAnN](https://help.galaxyproject.org/t/metatranscriptomic-analysis-fails-to-determine-pathwasy-with-humann/18426)
**Author:** [@jenny.gamezroessler](https://help.galaxyproject.org/u/jenny.gamezroessler)\ **Replies:** 3\ **Last updated:** [October 3, 2026, 12:29am UTC](https://help.galaxyproject.org/t/metatranscriptomic-analysis-fails-to-determine-pathwasy-with-humann/18426 "2026-10-03T00:29:48Z")
Hello Galaxy team, i write because i have a problem with the HUMAnN tool (v3.9 + galaxy1) i used on fecal NGS sequences (samples from four days). I have followed the tutorial “Metatranscriptomics analysis using microbio… --- ## [Trimmomatic error in variant analysis tutorial](https://help.galaxyproject.org/t/trimmomatic-error-in-variant-analysis-tutorial/18423)
**Author:** [@Adam\_Haberman](https://help.galaxyproject.org/u/Adam_Haberman)\ **Replies:** 3\ **Last updated:** [October 2, 2026, 11:30pm UTC](https://help.galaxyproject.org/t/trimmomatic-error-in-variant-analysis-tutorial/18423 "2026-10-02T23:30:05Z")
I’m pretty new to Galaxy, so I can’t tell if this error is my fault or not. I am working through the tutorial Identification of somatic and germline variants from tumor and normal sample pairs on usegalaxy. org (The hel… --- ## [Fatal Erreur Exit code 1 sur xcms findChromPeaks (v3.12.0+galaxy4)](https://help.galaxyproject.org/t/fatal-erreur-exit-code-1-sur-xcms-findchrompeaks-v3-12-0-galaxy4/18427)
**Author:** [@Theo](https://help.galaxyproject.org/u/Theo)\ **Replies:** 3\ **Last updated:** [October 1, 2026, 9:58am UTC](https://help.galaxyproject.org/t/fatal-erreur-exit-code-1-sur-xcms-findchrompeaks-v3-12-0-galaxy4/18427 "2026-10-01T09:58:16Z")
Hello, I am writing to report a systematic error when running a job on the Workflow4Metabolomics platform. The xcms findChromPeaks (xcmsSet) tool repeatedly fails during chromatographic peak detection. Job details: … --- ## [Which COI primers are suitable for mylabris pustulata DNA barcoding?](https://help.galaxyproject.org/t/which-coi-primers-are-suitable-for-mylabris-pustulata-dna-barcoding/18421)
**Author:** [@Hina\_Bibi](https://help.galaxyproject.org/u/Hina_Bibi)\ **Replies:** 1\ **Last updated:** [October 1, 2026, 12:13am UTC](https://help.galaxyproject.org/t/which-coi-primers-are-suitable-for-mylabris-pustulata-dna-barcoding/18421 "2026-10-01T00:13:58Z")
I am planning COI DNA barcoding of Mylabris pustulata and would appreciate advice from researchers working with Mylabris or Meloidae. I am considering either the universal COI primers LCO1490/HCO2198 or a universal forw… --- ## [Finding the MultiQC tool](https://help.galaxyproject.org/t/finding-the-multiqc-tool/18429)
**Author:** [@milaisabellebolger](https://help.galaxyproject.org/u/milaisabellebolger)\ **Replies:** 5\ **Last updated:** [September 30, 2026, 11:43pm UTC](https://help.galaxyproject.org/t/finding-the-multiqc-tool/18429 "2026-09-30T23:43:40Z")
Dear Galaxy EU, I am not able to find the MultiQC tool anymore. I used it before, but it does not appear anymore at my tools. Is there any reason I cannot use this anymore? I would like to use the MultiQC to put my many… --- ## [Requested memory issues](https://help.galaxyproject.org/t/requested-memory-issues/18430)
**Author:** [@bsegers](https://help.galaxyproject.org/u/bsegers)\ **Replies:** 1\ **Last updated:** [September 30, 2026, 4:30pm UTC](https://help.galaxyproject.org/t/requested-memory-issues/18430 "2026-09-30T16:30:05Z")
It’s been three weeks now that I haven’t been able to get any results using the GetOrganelle tool : Get organelle from reads (version 1.7.7.1+galaxy0). I keep getting the same error message about the requested memory. "… --- ## [Panaroo 1.8.0+galaxy0 fails on GFF3 collection with FileNotFoundError: input\_directory/\*.gff](https://help.galaxyproject.org/t/panaroo-1-8-0-galaxy0-fails-on-gff3-collection-with-filenotfounderror-input-directory-gff/18269)
**Author:** [@Loc\_Le](https://help.galaxyproject.org/u/Loc_Le)\ **Replies:** 7\ **Last updated:** [September 28, 2026, 5:59pm UTC](https://help.galaxyproject.org/t/panaroo-1-8-0-galaxy0-fails-on-gff3-collection-with-filenotfounderror-input-directory-gff/18269 "2026-09-28T17:59:43Z")
Hello, I encountered what appears to be a wrapper issue in Panaroo (Galaxy wrapper version 1.8.0+galaxy0 on usegalaxy.eu). Input: - Collection of 19 GFF3 files - All files were generated by Bakta - Collection dataty… --- ## [Reference genome for Drosophila issue](https://help.galaxyproject.org/t/reference-genome-for-drosophila-issue/18410)
**Author:** [@Maksym\_Dankovskyy](https://help.galaxyproject.org/u/Maksym_Dankovskyy)\ **Replies:** 7\ **Last updated:** [September 28, 2026, 5:41pm UTC](https://help.galaxyproject.org/t/reference-genome-for-drosophila-issue/18410 "2026-09-28T17:41:03Z")
Hello, I am trying to use RNA-STAR but I don’t believe the proper drosophila genome annotation is available under “built-in reference genome”. The two options are DM3 and release 6 ISO1 MT. However, I am looking specific… --- ## [Unable to save dataset attributes](https://help.galaxyproject.org/t/unable-to-save-dataset-attributes/18416)
**Author:** [@bsegers](https://help.galaxyproject.org/u/bsegers)\ **Replies:** 1\ **Last updated:** [September 24, 2026, 5:08pm UTC](https://help.galaxyproject.org/t/unable-to-save-dataset-attributes/18416 "2026-09-24T17:08:09Z")
I’m trying to change the format of a dataset obtained with AdapterRemoval. When I want to assign a new datatype from fastaqsanger to fasta, I’ve got the message : unable to save dataset attributes. I did this modificatio… --- ## [BEAST2 tool fails with ClassNotFoundException on Galaxy](https://help.galaxyproject.org/t/beast2-tool-fails-with-classnotfoundexception-on-galaxy/18414)
**Author:** [@Sawar](https://help.galaxyproject.org/u/Sawar)\ **Replies:** 1\ **Last updated:** [September 23, 2026, 6:08pm UTC](https://help.galaxyproject.org/t/beast2-tool-fails-with-classnotfoundexception-on-galaxy/18414 "2026-09-23T18:08:55Z")
Continuing the discussion from Installation of BEAST on Galaxy Europe: Dear Galaxy Europe team, I’m trying to run the BEAST2 tool on usegalaxy.eu and the job fails immediately, before the analysis starts. The same XML … --- ## [Galaxy server slow and freezed](https://help.galaxyproject.org/t/galaxy-server-slow-and-freezed/18411)
**Author:** [@karenandreac](https://help.galaxyproject.org/u/karenandreac)\ **Replies:** 1\ **Last updated:** [September 23, 2026, 5:21pm UTC](https://help.galaxyproject.org/t/galaxy-server-slow-and-freezed/18411 "2026-09-23T17:21:15Z")
Hello! Since yesterday I’ve been working with DADA2 in Galaxy.org and it has been very slow, the tool MakeSequenceTable has been freezed without initiating for many hours now. Would you let me know if there are any iss… --- ## [Direct transfer of ~109 GB Novogene RNA-seq data to UseGalaxy.eu and recommended storage](https://help.galaxyproject.org/t/direct-transfer-of-109-gb-novogene-rna-seq-data-to-usegalaxy-eu-and-recommended-storage/18390)
**Author:** [@aaak](https://help.galaxyproject.org/u/aaak)\ **Replies:** 5\ **Last updated:** [September 22, 2026, 12:44pm UTC](https://help.galaxyproject.org/t/direct-transfer-of-109-gb-novogene-rna-seq-data-to-usegalaxy-eu-and-recommended-storage/18390 "2026-09-22T12:44:44Z")
Dear Galaxy community, Following a suggestion from the Galaxy Europe support team, I am posting our question here so that the answer may also be useful to other users. We have a paired-end mRNA-seq dataset consisting o… --- ## [UseGalaxy.fr service updates re: IFB Core Cluster infrastructure shutdown from September 14th to 21st, 2026](https://help.galaxyproject.org/t/usegalaxy-fr-service-updates-re-ifb-core-cluster-infrastructure-shutdown-from-september-14th-to-21st-2026/18409)
**Author:** [@tristan.lefebure](https://help.galaxyproject.org/u/tristan.lefebure)\ **Replies:** 1\ **Last updated:** [September 21, 2026, 7:09pm UTC](https://help.galaxyproject.org/t/usegalaxy-fr-service-updates-re-ifb-core-cluster-infrastructure-shutdown-from-september-14th-to-21st-2026/18409 "2026-09-21T19:09:20Z")
Dear all, Is installing FROGS on usegalaxy\_eu still not possible ? usegalaxy\_fr as an electrical issue, and I can’t find an other alternative… and my training session is next week ;( Thanks --- ## [Installation of FROGS (metagenomics from metabarcoding.usegalaxy.fr) on usegalaxy.eu](https://help.galaxyproject.org/t/installation-of-frogs-metagenomics-from-metabarcoding-usegalaxy-fr-on-usegalaxy-eu/16225)
**Author:** [@andy\_laprie](https://help.galaxyproject.org/u/andy_laprie)\ **Replies:** 2\ **Last updated:** [August 29, 2025, 8:36pm UTC](https://help.galaxyproject.org/t/installation-of-frogs-metagenomics-from-metabarcoding-usegalaxy-fr-on-usegalaxy-eu/16225 "2025-08-29T20:36:54Z")
Hello, Would it be possible to install the metabarcoding tool FROGS on my Galaxy.eu session please? Thank you very much in advance ! AL --- ## [UseGalaxy.org account activation required — no activation email received](https://help.galaxyproject.org/t/usegalaxy-org-account-activation-required-no-activation-email-received/18406)
**Author:** [@Yamini\_chandel](https://help.galaxyproject.org/u/Yamini_chandel)\ **Replies:** 1\ **Last updated:** [September 21, 2026, 6:44pm UTC](https://help.galaxyproject.org/t/usegalaxy-org-account-activation-required-no-activation-email-received/18406 "2026-09-21T18:44:47Z")
Hello Galaxy Support, I am using UseGalaxy.org and I am getting the error: “Action requires account activation.” I am already logged into my account and I have successfully completed several steps in my Galaxy history… --- ## [Revival of login bug](https://help.galaxyproject.org/t/revival-of-login-bug/18382)
**Author:** [@sukasuru](https://help.galaxyproject.org/u/sukasuru)\ **Replies:** 1\ **Last updated:** [September 14, 2026, 6:12pm UTC](https://help.galaxyproject.org/t/revival-of-login-bug/18382 "2026-09-14T18:12:13Z")
Hi, an issue last logged here in 2023 reemerged where upon trying to enter in the reset password link into my browser for the panel view, it defaults back to the login page. In fact the link isn’t being registered as a l… --- ## [Funannotate BUSCO models - mucorales missing](https://help.galaxyproject.org/t/funannotate-busco-models-mucorales-missing/18386)
**Author:** [@Sydney\_Harned](https://help.galaxyproject.org/u/Sydney_Harned)\ **Replies:** 1\ **Last updated:** [September 14, 2026, 5:54pm UTC](https://help.galaxyproject.org/t/funannotate-busco-models-mucorales-missing/18386 "2026-09-14T17:54:43Z")
Hi Galaxy team, I’m running the “Funannotate predict annotation” tool and the “BUSCO models to align” dropdown only shows Dikarya as an option. Could you please add mucorales\_odb10 to the available lineages for this too… --- ## [I can´t activated my acount](https://help.galaxyproject.org/t/i-can-t-activated-my-acount/18381)
**Author:** [@Maji\_Dalu](https://help.galaxyproject.org/u/Maji_Dalu)\ **Replies:** 1\ **Last updated:** [September 14, 2026, 3:11pm UTC](https://help.galaxyproject.org/t/i-can-t-activated-my-acount/18381 "2026-09-14T15:11:41Z")
I never received the activation email --- ## [Scheduled downtime due to data center power test on September 15](https://help.galaxyproject.org/t/scheduled-downtime-due-to-data-center-power-test-on-september-15/18384)
**Author:** [@team-freiburg](https://help.galaxyproject.org/u/team-freiburg)\ **Replies:** 0\ **Last updated:** [September 14, 2026, 9:42am UTC](https://help.galaxyproject.org/t/scheduled-downtime-due-to-data-center-power-test-on-september-15/18384 "2026-09-14T09:42:11Z")
Due to a scheduled data center power cut and recovery test, there will be a downtime on 15th September, from 7:30 a.m. to at least 10:30 a.m. CEST. This test will improve our data center’s resilience against power outage… --- ## [Failed to betaupload](https://help.galaxyproject.org/t/failed-to-betaupload/18377)
**Author:** [@arbv](https://help.galaxyproject.org/u/arbv)\ **Replies:** 1\ **Last updated:** [September 11, 2026, 8:06pm UTC](https://help.galaxyproject.org/t/failed-to-betaupload/18377 "2026-09-11T20:06:35Z")
Server: usegalaxy.org Upload method: Import Data (Beta) File: MDMS240000022-3a\_L1\_1.fq.gz Size: 6.1 GB Target history: SJ29\_assembly\_mapping\_BWA Upload Progress shows 100% completed, but the dataset does not appear … --- ## [Requesting Activation of FTP](https://help.galaxyproject.org/t/requesting-activation-of-ftp/18379)
**Author:** [@ephillippi](https://help.galaxyproject.org/u/ephillippi)\ **Replies:** 1\ **Last updated:** [September 10, 2026, 6:22pm UTC](https://help.galaxyproject.org/t/requesting-activation-of-ftp/18379 "2026-09-10T18:22:13Z")
I am trying to upload 100-300GB files for analysis. The upload has failed multiple times in the web uploader. I really need to be able to restart an upload when there are network hiccups, but FTP is not an option availab… --- ## [Data Upload delays UseGalaxy.org Sept 2026](https://help.galaxyproject.org/t/data-upload-delays-usegalaxy-org-sept-2026/18375)
**Author:** [@Linda\_Kaira](https://help.galaxyproject.org/u/Linda_Kaira)\ **Replies:** 3\ **Last updated:** [September 10, 2026, 4:45pm UTC](https://help.galaxyproject.org/t/data-upload-delays-usegalaxy-org-sept-2026/18375 "2026-09-10T16:45:50Z")
Good day, I have an issue of my data having queued for over 24 hours and even if I upload new data I am still receiving a queued status. I have even started a new history but it is still giving me a queued status --- ## [How to run VGP workflow with large files?](https://help.galaxyproject.org/t/how-to-run-vgp-workflow-with-large-files/18368)
**Author:** [@jinglin](https://help.galaxyproject.org/u/jinglin)\ **Replies:** 2\ **Last updated:** [September 9, 2026, 6:30am UTC](https://help.galaxyproject.org/t/how-to-run-vgp-workflow-with-large-files/18368 "2026-09-09T06:30:20Z")
I’m trying to run VGP workflow for my genome assembly, but my HiFi data is large which is 230GB. It is about 60GB in compressed (.gz) format, but VGP workflow only accept uncompressed files. My total storage size is only… --- ## [Jobs queues UseGalaxy.eu August 2026](https://help.galaxyproject.org/t/jobs-queues-usegalaxy-eu-august-2026/18289)
**Author:** [@JGojznikar](https://help.galaxyproject.org/u/JGojznikar)\ **Replies:** 6\ **Last updated:** [September 7, 2026, 9:53pm UTC](https://help.galaxyproject.org/t/jobs-queues-usegalaxy-eu-august-2026/18289 "2026-09-07T21:53:50Z")
Hello everyone, I’ve currently scheduled a test workflow run on a subset of samples. The workflow passed previous runs without any clear issue, however now has been on scheduled (“pending”) for more than two weeks. On a… --- ## [Confirmed and pending status: Tool "Retrieve bold" is not working : warning message + log](https://help.galaxyproject.org/t/confirmed-and-pending-status-tool-retrieve-bold-is-not-working-warning-message-log/18369)
**Author:** [@eline.jkn](https://help.galaxyproject.org/u/eline.jkn)\ **Replies:** 2\ **Last updated:** [September 7, 2026, 9:37pm UTC](https://help.galaxyproject.org/t/confirmed-and-pending-status-tool-retrieve-bold-is-not-working-warning-message-log/18369 "2026-09-07T21:37:39Z")
Does not work, always same error: Tool standard error : Warning message: In data.table::fread(text = x, header = header, sep = sep, ...) : Stopped early on line 44. Expected 2 fields but found 0. Consider fill=TRUE a… --- ## [Prodigal Gene Predictor: error option (# Other genetic codes 1-25) stderr Invalid translation table specified](https://help.galaxyproject.org/t/prodigal-gene-predictor-error-option-other-genetic-codes-1-25-stderr-invalid-translation-table-specified/18350)
**Author:** [@tvtv195](https://help.galaxyproject.org/u/tvtv195)\ **Replies:** 1\ **Last updated:** [September 7, 2026, 5:55pm UTC](https://help.galaxyproject.org/t/prodigal-gene-predictor-error-option-other-genetic-codes-1-25-stderr-invalid-translation-table-specified/18350 "2026-09-07T17:55:12Z")
I am experiencing a bug in this specific version of the Galaxy wrapper (Galaxy Version 2.6.3+galaxy0). The developer forgot to link the conditional text field container to the # : Other genetic codes 1-25 selection choi… [Next page](https://help.galaxyproject.org/latest.md?no_subcategories=false&page=1)