# Latest **URL:** https://help.galaxyproject.org/latest.md?no_subcategories=false&page=2 [Latest](https://help.galaxyproject.org/latest.md) · [Categories](https://help.galaxyproject.org/categories.md) · [Tags](https://help.galaxyproject.org/tags.md) **Page:** 3 --- ## ["Invalid storage location" when trying to move data to Backblaze B2](https://help.galaxyproject.org/t/invalid-storage-location-when-trying-to-move-data-to-backblaze-b2/18276)
**Author:** [@Peter\_van\_Heusden](https://help.galaxyproject.org/u/Peter_van_Heusden)\ **Replies:** 5\ **Last updated:** [August 12, 2026, 10:30am UTC](https://help.galaxyproject.org/t/invalid-storage-location-when-trying-to-move-data-to-backblaze-b2/18276 "2026-08-12T10:30:55Z")
I’m trying to move the datasets in a history (8228344521d4e949) to my Backblaze B2 storage but when I try and do this with the “Manage storage location” option, I get told that “The selected storage location is not avail… --- ## [ABRicate databases and tool versions in workflows](https://help.galaxyproject.org/t/abricate-databases-and-tool-versions-in-workflows/18216)
**Author:** [@Sarah\_Teichman](https://help.galaxyproject.org/u/Sarah_Teichman)\ **Replies:** 3\ **Last updated:** [August 11, 2026, 6:31pm UTC](https://help.galaxyproject.org/t/abricate-databases-and-tool-versions-in-workflows/18216 "2026-08-11T18:31:10Z")
Hi, I would like to use ABRicate with the bacmet2 database, and when I run ABRicate List in Galaxy it lists 12 available databases, including bacmet2, but when I look at the workflow for ABRicate itself, under advanced o… --- ## [Exporting OTU/ASV and Taxonomy tables (BIOM / CSV) for MicrobiomeAnalyst from Nanopore 16S GTN Tutorial](https://help.galaxyproject.org/t/exporting-otu-asv-and-taxonomy-tables-biom-csv-for-microbiomeanalyst-from-nanopore-16s-gtn-tutorial/18262)
**Author:** [@zahir](https://help.galaxyproject.org/u/zahir)\ **Replies:** 3\ **Last updated:** [August 11, 2026, 6:23pm UTC](https://help.galaxyproject.org/t/exporting-otu-asv-and-taxonomy-tables-biom-csv-for-microbiomeanalyst-from-nanopore-16s-gtn-tutorial/18262 "2026-08-11T18:23:08Z")
Hello Galaxy Community, I am conducting bacterial diversity analysis on sediment samples using the Oxford Nanopore MinION platform for 16S sequencing. Basecalling and demultiplexing were performed using MinKNOW. After … --- ## [Notebooks: more about memory resources and HTP analysis](https://help.galaxyproject.org/t/notebooks-more-about-memory-resources-and-htp-analysis/18267)
**Author:** [@0808](https://help.galaxyproject.org/u/0808)\ **Replies:** 2\ **Last updated:** [August 11, 2026, 7:31am UTC](https://help.galaxyproject.org/t/notebooks-more-about-memory-resources-and-htp-analysis/18267 "2026-08-11T07:31:33Z")
Title: Request for higher-memory resource for RSEM v1.3.1 due to OOM Hello Galaxy Europe Support Team, I am reprocessing the public PRJEB40416 gastric cancer RNA-seq dataset on usegalaxy.eu. STAR alignment has comple… --- ## [Troubleshooting: temporary file limitation breaking Orthofinder? Known in original tool](https://help.galaxyproject.org/t/troubleshooting-temporary-file-limitation-breaking-orthofinder-known-in-original-tool/18054)
**Author:** [@imarimas](https://help.galaxyproject.org/u/imarimas)\ **Replies:** 2\ **Last updated:** [August 10, 2026, 9:04pm UTC](https://help.galaxyproject.org/t/troubleshooting-temporary-file-limitation-breaking-orthofinder-known-in-original-tool/18054 "2026-08-10T21:04:11Z")
Greetings, i am currently running a comparative genomics analysis on several genomes (10 to be exact) using protein fasta in OrthoFinder after normalization, the jobs have been running for almost/over two days now with … --- ## [Interactive JupyterLab Notebook - use terminal for RNA-seq alignment](https://help.galaxyproject.org/t/interactive-jupyterlab-notebook-use-terminal-for-rna-seq-alignment/18055)
**Author:** [@cventophd](https://help.galaxyproject.org/u/cventophd)\ **Replies:** 1\ **Last updated:** [August 10, 2026, 8:19pm UTC](https://help.galaxyproject.org/t/interactive-jupyterlab-notebook-use-terminal-for-rna-seq-alignment/18055 "2026-08-10T20:19:06Z")
Hello, I would like to try an experiment where I use the CLI in Galaxy via Interactive JupyterLab Notebook to do an RNA-seq alignment using histat2 or STAR. However, whenever I use either these in the terminal I get “co… --- ## [Busco string\_type error](https://help.galaxyproject.org/t/busco-string-type-error/18060)
**Author:** [@Joseph\_Kogan](https://help.galaxyproject.org/u/Joseph_Kogan)\ **Replies:** 1\ **Last updated:** [August 10, 2026, 7:17pm UTC](https://help.galaxyproject.org/t/busco-string-type-error/18060 "2026-08-10T19:17:43Z")
I am trying to run BUSCO on a protein fasta file generated from a gff3 annotation, which has worked without a hitch in the past. This time, no matter what I do and regardless of the fact that I am using the exact same to… --- ## [Featurecounts: reads not mapping to annotated features](https://help.galaxyproject.org/t/featurecounts-reads-not-mapping-to-annotated-features/18051)
**Author:** [@bahar125d](https://help.galaxyproject.org/u/bahar125d)\ **Replies:** 3\ **Last updated:** [August 10, 2026, 7:15pm UTC](https://help.galaxyproject.org/t/featurecounts-reads-not-mapping-to-annotated-features/18051 "2026-08-10T19:15:11Z")
Hello, I am facing a very similar issue here analyzing my bulk RNA-seq data from 6 samples. I trimmed the adapters and ran FastQC on my samples. I ran HISAT2 and FeatureCounts. I got very high %alignment for HISAT2 acc… --- ## [GTDB-Tk, reference data, and job queues: leave jobs queued for this competitive cluster group!](https://help.galaxyproject.org/t/gtdb-tk-reference-data-and-job-queues-leave-jobs-queued-for-this-competitive-cluster-group/18237)
**Author:** [@horikini](https://help.galaxyproject.org/u/horikini)\ **Replies:** 3\ **Last updated:** [August 10, 2026, 6:21pm UTC](https://help.galaxyproject.org/t/gtdb-tk-reference-data-and-job-queues-leave-jobs-queued-for-this-competitive-cluster-group/18237 "2026-08-10T18:21:52Z")
Hi, I have a GTDB-Tk classify\_wf job that has been stuck in “queued” state (never transitioned to “running”) for over 73 hours. Job ID: 4838ba20a6d86765aad1b932f8f80dd1 Tool: toolshed.g2.bx.psu.edu/repos/iuc/gtdbtk\_cl… --- ## [Teaching school children eDNA bioinformatics (sequence files to Krona chart) in 30 minutes](https://help.galaxyproject.org/t/teaching-school-children-edna-bioinformatics-sequence-files-to-krona-chart-in-30-minutes/18257)
**Author:** [@Adam\_Hillier](https://help.galaxyproject.org/u/Adam_Hillier)\ **Replies:** 4\ **Last updated:** [August 10, 2026, 6:17pm UTC](https://help.galaxyproject.org/t/teaching-school-children-edna-bioinformatics-sequence-files-to-krona-chart-in-30-minutes/18257 "2026-08-10T18:17:57Z")
I have prepared an online teach-the-schoolteacher eDNA demo. The teacher is guided to build and run the following eDNA bioinformatics work flow from scratch in 30min. The teacher is then able to teach his/her school stud… --- ## [Unable to access Galaxy (Connection timed out)](https://help.galaxyproject.org/t/unable-to-access-galaxy-connection-timed-out/18254)
**Author:** [@noamesh11](https://help.galaxyproject.org/u/noamesh11)\ **Replies:** 1\ **Last updated:** [August 7, 2026, 5:02pm UTC](https://help.galaxyproject.org/t/unable-to-access-galaxy-connection-timed-out/18254 "2026-08-07T17:02:02Z")
Hello, I am unable to access the Galaxy server at https://galaxy.biobakery.org in order to continue my MaAsLin2 analysis. Whenever I try to open the website, I receive the following error: ERR\_CONNECTION\_TIMED\_OUT I … --- ## [Google Drive repository connection](https://help.galaxyproject.org/t/google-drive-repository-connection/18223)
**Author:** [@Joseph\_Kogan](https://help.galaxyproject.org/u/Joseph_Kogan)\ **Replies:** 1\ **Last updated:** [August 6, 2026, 10:19pm UTC](https://help.galaxyproject.org/t/google-drive-repository-connection/18223 "2026-08-06T22:19:59Z")
Hello! I am trying to connect my Galaxy account directly to Google Drive as a repository in the hopes that it will make uploading multi-GB files faster… I am repeatedly running into the error message attached. Would appr… --- ## [Jobs queues: how to check the server stats at UseGalaxy.eu](https://help.galaxyproject.org/t/jobs-queues-how-to-check-the-server-stats-at-usegalaxy-eu/18251)
**Author:** [@mokba312](https://help.galaxyproject.org/u/mokba312)\ **Replies:** 2\ **Last updated:** [August 6, 2026, 5:10pm UTC](https://help.galaxyproject.org/t/jobs-queues-how-to-check-the-server-stats-at-usegalaxy-eu/18251 "2026-08-06T17:10:52Z")
I am getting same problem don’ know what is the issue --- ## [Jobs remain in "new" state since Tuesday maintenance on usegalaxy.eu](https://help.galaxyproject.org/t/jobs-remain-in-new-state-since-tuesday-maintenance-on-usegalaxy-eu/18249)
**Author:** [@ehmig](https://help.galaxyproject.org/u/ehmig)\ **Replies:** 1\ **Last updated:** [August 6, 2026, 4:59pm UTC](https://help.galaxyproject.org/t/jobs-remain-in-new-state-since-tuesday-maintenance-on-usegalaxy-eu/18249 "2026-08-06T16:59:01Z")
Hello, I’m experiencing an issue on usegalaxy.eu since the scheduled maintenance on Tuesday at around 14:00 (CEST). Before the maintenance, everything was working normally. Since then, I have not been able to run any j… --- ## [Abnormally long-running BRAKER3 job on usegalaxy.eu](https://help.galaxyproject.org/t/abnormally-long-running-braker3-job-on-usegalaxy-eu/18244)
**Author:** [@lupolk](https://help.galaxyproject.org/u/lupolk)\ **Replies:** 2\ **Last updated:** [August 6, 2026, 2:32pm UTC](https://help.galaxyproject.org/t/abnormally-long-running-braker3-job-on-usegalaxy-eu/18244 "2026-08-06T14:32:06Z")
Hi, I have a BRAKER3 3.0.8+galaxy2 job that has been running since 14 July 2026. A previous submission of the same analysis remained running for more around a month until I cancelled it. Comparable BRAKER3 analyses for … --- ## [Jobs waiting to run](https://help.galaxyproject.org/t/jobs-waiting-to-run/9623)
**Author:** [@christkr08](https://help.galaxyproject.org/u/christkr08)\ **Replies:** 8\ **Last updated:** [June 4, 2023, 2:22pm UTC](https://help.galaxyproject.org/t/jobs-waiting-to-run/9623 "2023-06-04T14:22:51Z")
Hi all, I have uploaded my WGS fq.gz files yesterday but they are still waiting to run… Anyone else with similar problems or with any suggestion? Thanks for any help. --- ## [Humann troubleshooting](https://help.galaxyproject.org/t/humann-troubleshooting/18235)
**Author:** [@Tahmine\_Aldaghi](https://help.galaxyproject.org/u/Tahmine_Aldaghi)\ **Replies:** 1\ **Last updated:** [August 6, 2026, 2:18am UTC](https://help.galaxyproject.org/t/humann-troubleshooting/18235 "2026-08-06T02:18:56Z")
Hi, I want to use Humann, but I frequently encounter a 504 error. What is the reason for this error? I would be grateful if someone could help me. --- ## [Diamond indexes: synchronizing across UseGalaxy servers](https://help.galaxyproject.org/t/diamond-indexes-synchronizing-across-usegalaxy-servers/18242)
**Author:** [@Osmannamal](https://help.galaxyproject.org/u/Osmannamal)\ **Replies:** 1\ **Last updated:** [August 5, 2026, 8:55pm UTC](https://help.galaxyproject.org/t/diamond-indexes-synchronizing-across-usegalaxy-servers/18242 "2026-08-05T20:55:27Z")
Hello, i am trying to use diamond blast tool on the galaxy platform. I need to use the most up-to-date NCBI database possible for protein blast. Ncbi reference database on the usegalaxy.eu is quite up to date (dated 2025… --- ## [Datamash across many columns](https://help.galaxyproject.org/t/datamash-across-many-columns/18240)
**Author:** [@rmassei](https://help.galaxyproject.org/u/rmassei)\ **Replies:** 1\ **Last updated:** [August 5, 2026, 8:49pm UTC](https://help.galaxyproject.org/t/datamash-across-many-columns/18240 "2026-08-05T20:49:28Z")
Hi! I’m trying to group by a column and compute the median and mean per group across many columns of a CSV file. Datamash seems like the tool for this task, but it looks like I can only select a single column to perfor… --- ## [Dropped tximeta singularity image in depot](https://help.galaxyproject.org/t/dropped-tximeta-singularity-image-in-depot/18215)
**Author:** [@noronhaa](https://help.galaxyproject.org/u/noronhaa)\ **Replies:** 1\ **Last updated:** [August 5, 2026, 9:14am UTC](https://help.galaxyproject.org/t/dropped-tximeta-singularity-image-in-depot/18215 "2026-08-05T09:14:06Z")
not sure if this is the right place to bring up this issue. the galaxy project depot for singularity images used to have the following images: bioconductor-tximeta:1.20.1–r43hdfd78af\_0 bioconductor-tximeta:1.20.1–r43hd… --- ## [Request to increase dataset selection limit](https://help.galaxyproject.org/t/request-to-increase-dataset-selection-limit/18230)
**Author:** [@ANA\_PINHEIRO](https://help.galaxyproject.org/u/ANA_PINHEIRO)\ **Replies:** 1\ **Last updated:** [August 4, 2026, 8:36pm UTC](https://help.galaxyproject.org/t/request-to-increase-dataset-selection-limit/18230 "2026-08-04T20:36:09Z")
Dear Galaxy Europe Team, I am a PhD student using Galaxy Europe for bacterial genomics analyses. I work with large datasets (more than 50 bacterial genomes) and routinely use Prokka followed by ABRicate for resistome, v… --- ## [Release of Galaxy 26.1](https://help.galaxyproject.org/t/release-of-galaxy-26-1/18236)
**Author:** [@natwhitaker](https://help.galaxyproject.org/u/natwhitaker)\ **Replies:** 0\ **Last updated:** [August 4, 2026, 4:28pm UTC](https://help.galaxyproject.org/t/release-of-galaxy-26-1/18236 "2026-08-04T16:28:43Z")
Dear Galaxy Community, The Galaxy Committers team is pleased to announce the Galaxy 26.1 release! Release Notes User release announcement Developer and admin release announcement GitHub Comprehensive Release No… --- ## [Scheduled Cluster Maintenance August 4-5 at UseGalaxy.org](https://help.galaxyproject.org/t/scheduled-cluster-maintenance-august-4-5-at-usegalaxy-org/18233)
**Author:** [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Replies:** 0\ **Last updated:** [August 3, 2026, 7:35pm UTC](https://help.galaxyproject.org/t/scheduled-cluster-maintenance-august-4-5-at-usegalaxy-org/18233 "2026-08-03T19:35:27Z")
:globe\_with\_meridians: UseGalaxy.org Scheduled Cluster Maintenance August 4-5 2026 The Jetstream2 cloud, which services a large number of Galaxy’s larger (high core/memory) jobs and Galaxy Interactive Tools will be offl… --- ## [Flexynesis tool failure - mkdir: mkdir: cannot create directory 'tmp': Permission denied error](https://help.galaxyproject.org/t/flexynesis-tool-failure-mkdir-mkdir-cannot-create-directory-tmp-permission-denied-error/18195)
**Author:** [@lordtsage](https://help.galaxyproject.org/u/lordtsage)\ **Replies:** 10\ **Last updated:** [August 3, 2026, 5:39pm UTC](https://help.galaxyproject.org/t/flexynesis-tool-failure-mkdir-mkdir-cannot-create-directory-tmp-permission-denied-error/18195 "2026-08-03T17:39:40Z")
mkdir: mkdir: cannot create directory ‘tmp’: Permission denied Server instance: UseGalaxy . eu --- ## [Not able to process software](https://help.galaxyproject.org/t/not-able-to-process-software/18219)
**Author:** [@Harshraj\_Solanki](https://help.galaxyproject.org/u/Harshraj_Solanki)\ **Replies:** 2\ **Last updated:** [July 31, 2026, 7:38pm UTC](https://help.galaxyproject.org/t/not-able-to-process-software/18219 "2026-07-31T19:38:08Z")
i am not able to use galaxy software i dont know why --- ## [Issue Using MIBiG with BiG-SCAPE on Galaxy](https://help.galaxyproject.org/t/issue-using-mibig-with-big-scape-on-galaxy/18099)
**Author:** [@Shreyash\_04](https://help.galaxyproject.org/u/Shreyash_04)\ **Replies:** 3\ **Last updated:** [July 31, 2026, 7:35pm UTC](https://help.galaxyproject.org/t/issue-using-mibig-with-big-scape-on-galaxy/18099 "2026-07-31T19:35:11Z")
Dear Galaxy Support Team, I hope you are doing well. I am experiencing persistent issues while running BiG-SCAPE on Galaxy, specifically related to the inclusion of the MIBiG reference dataset. :red\_circle: MIBiG issu… --- ## [Interactive tools not working](https://help.galaxyproject.org/t/interactive-tools-not-working/18225)
**Author:** [@Chris\_Tsoukas](https://help.galaxyproject.org/u/Chris_Tsoukas)\ **Replies:** 1\ **Last updated:** [July 31, 2026, 10:36am UTC](https://help.galaxyproject.org/t/interactive-tools-not-working/18225 "2026-07-31T10:36:56Z")
Hello! I was using the JupyTool interactive tool and it worked pefectly. All of a sudden, when I am trying to load a previous notebook, or even start a new session, the three jobs are in queue (gray color, says this job … --- ## [MrBayes consensus tree file not shown in output](https://help.galaxyproject.org/t/mrbayes-consensus-tree-file-not-shown-in-output/18221)
**Author:** [@tavovato](https://help.galaxyproject.org/u/tavovato)\ **Replies:** 2\ **Last updated:** [July 30, 2026, 9:52pm UTC](https://help.galaxyproject.org/t/mrbayes-consensus-tree-file-not-shown-in-output/18221 "2026-07-30T21:52:26Z")
Hi! I ran a MrBayes analysis on usegalaxy.eu and the job seems to have completed successfully. The summary report indicates that several MrBayes output files were generated, including .run1.t, .run2.t, .pstat, .tstat, .… --- ## [WGCNA Galaxy Tool Error: nThreads must be numeric and at least 2](https://help.galaxyproject.org/t/wgcna-galaxy-tool-error-nthreads-must-be-numeric-and-at-least-2/18199)
**Author:** [@konidala\_bio](https://help.galaxyproject.org/u/konidala_bio)\ **Replies:** 3\ **Last updated:** [July 30, 2026, 6:19pm UTC](https://help.galaxyproject.org/t/wgcna-galaxy-tool-error-nthreads-must-be-numeric-and-at-least-2/18199 "2026-07-30T18:19:46Z")
Dear Galaxy Support Team, I am using the WGCNA Network Galaxy tool with the following Tool ID: toolshed.g2.bx.psu.edu/repos/iuc/wgcna\_network/wgcna\_network/1.74+galaxy0 My job is failing with the following error: Err… --- ## [MrBayes - no output files found](https://help.galaxyproject.org/t/mrbayes-no-output-files-found/16275)
**Author:** [@Alan\_Thomas](https://help.galaxyproject.org/u/Alan_Thomas)\ **Replies:** 4\ **Last updated:** [July 30, 2026, 6:12pm UTC](https://help.galaxyproject.org/t/mrbayes-no-output-files-found/16275 "2026-07-30T18:12:31Z")
Hai After analysing data using MrBayes in https://usegalaxy.eu/, output files are not created. No output files found. Only a text file was found. Could you please help me? Thank you. [Previous page](https://help.galaxyproject.org/latest.md?no_subcategories=false&page=1) [Next page](https://help.galaxyproject.org/latest.md?no_subcategories=false&page=3)