# Latest **URL:** https://help.galaxyproject.org/latest.md?page=2 [Latest](https://help.galaxyproject.org/latest.md) · [Categories](https://help.galaxyproject.org/categories.md) · [Tags](https://help.galaxyproject.org/tags.md) **Page:** 3 --- ## [Galaxy server slow and freezed](https://help.galaxyproject.org/t/galaxy-server-slow-and-freezed/18411)
**Author:** [@karenandreac](https://help.galaxyproject.org/u/karenandreac)\ **Replies:** 1\ **Last updated:** [September 23, 2026, 5:21pm UTC](https://help.galaxyproject.org/t/galaxy-server-slow-and-freezed/18411 "2026-09-23T17:21:15Z")
Hello! Since yesterday I’ve been working with DADA2 in Galaxy.org and it has been very slow, the tool MakeSequenceTable has been freezed without initiating for many hours now. Would you let me know if there are any iss… --- ## [Direct transfer of ~109 GB Novogene RNA-seq data to UseGalaxy.eu and recommended storage](https://help.galaxyproject.org/t/direct-transfer-of-109-gb-novogene-rna-seq-data-to-usegalaxy-eu-and-recommended-storage/18390)
**Author:** [@aaak](https://help.galaxyproject.org/u/aaak)\ **Replies:** 5\ **Last updated:** [September 22, 2026, 12:44pm UTC](https://help.galaxyproject.org/t/direct-transfer-of-109-gb-novogene-rna-seq-data-to-usegalaxy-eu-and-recommended-storage/18390 "2026-09-22T12:44:44Z")
Dear Galaxy community, Following a suggestion from the Galaxy Europe support team, I am posting our question here so that the answer may also be useful to other users. We have a paired-end mRNA-seq dataset consisting o… --- ## [UseGalaxy.fr service updates re: IFB Core Cluster infrastructure shutdown from September 14th to 21st, 2026](https://help.galaxyproject.org/t/usegalaxy-fr-service-updates-re-ifb-core-cluster-infrastructure-shutdown-from-september-14th-to-21st-2026/18409)
**Author:** [@tristan.lefebure](https://help.galaxyproject.org/u/tristan.lefebure)\ **Replies:** 1\ **Last updated:** [September 21, 2026, 7:09pm UTC](https://help.galaxyproject.org/t/usegalaxy-fr-service-updates-re-ifb-core-cluster-infrastructure-shutdown-from-september-14th-to-21st-2026/18409 "2026-09-21T19:09:20Z")
Dear all, Is installing FROGS on usegalaxy\_eu still not possible ? usegalaxy\_fr as an electrical issue, and I can’t find an other alternative… and my training session is next week ;( Thanks --- ## [Installation of FROGS (metagenomics from metabarcoding.usegalaxy.fr) on usegalaxy.eu](https://help.galaxyproject.org/t/installation-of-frogs-metagenomics-from-metabarcoding-usegalaxy-fr-on-usegalaxy-eu/16225)
**Author:** [@andy\_laprie](https://help.galaxyproject.org/u/andy_laprie)\ **Replies:** 2\ **Last updated:** [August 29, 2025, 8:36pm UTC](https://help.galaxyproject.org/t/installation-of-frogs-metagenomics-from-metabarcoding-usegalaxy-fr-on-usegalaxy-eu/16225 "2025-08-29T20:36:54Z")
Hello, Would it be possible to install the metabarcoding tool FROGS on my Galaxy.eu session please? Thank you very much in advance ! AL --- ## [UseGalaxy.org account activation required — no activation email received](https://help.galaxyproject.org/t/usegalaxy-org-account-activation-required-no-activation-email-received/18406)
**Author:** [@Yamini\_chandel](https://help.galaxyproject.org/u/Yamini_chandel)\ **Replies:** 1\ **Last updated:** [September 21, 2026, 6:44pm UTC](https://help.galaxyproject.org/t/usegalaxy-org-account-activation-required-no-activation-email-received/18406 "2026-09-21T18:44:47Z")
Hello Galaxy Support, I am using UseGalaxy.org and I am getting the error: “Action requires account activation.” I am already logged into my account and I have successfully completed several steps in my Galaxy history… --- ## [Revival of login bug](https://help.galaxyproject.org/t/revival-of-login-bug/18382)
**Author:** [@sukasuru](https://help.galaxyproject.org/u/sukasuru)\ **Replies:** 1\ **Last updated:** [September 14, 2026, 6:12pm UTC](https://help.galaxyproject.org/t/revival-of-login-bug/18382 "2026-09-14T18:12:13Z")
Hi, an issue last logged here in 2023 reemerged where upon trying to enter in the reset password link into my browser for the panel view, it defaults back to the login page. In fact the link isn’t being registered as a l… --- ## [Funannotate BUSCO models - mucorales missing](https://help.galaxyproject.org/t/funannotate-busco-models-mucorales-missing/18386)
**Author:** [@Sydney\_Harned](https://help.galaxyproject.org/u/Sydney_Harned)\ **Replies:** 1\ **Last updated:** [September 14, 2026, 5:54pm UTC](https://help.galaxyproject.org/t/funannotate-busco-models-mucorales-missing/18386 "2026-09-14T17:54:43Z")
Hi Galaxy team, I’m running the “Funannotate predict annotation” tool and the “BUSCO models to align” dropdown only shows Dikarya as an option. Could you please add mucorales\_odb10 to the available lineages for this too… --- ## [I can´t activated my acount](https://help.galaxyproject.org/t/i-can-t-activated-my-acount/18381)
**Author:** [@Maji\_Dalu](https://help.galaxyproject.org/u/Maji_Dalu)\ **Replies:** 1\ **Last updated:** [September 14, 2026, 3:11pm UTC](https://help.galaxyproject.org/t/i-can-t-activated-my-acount/18381 "2026-09-14T15:11:41Z")
I never received the activation email --- ## [Scheduled downtime due to data center power test on September 15](https://help.galaxyproject.org/t/scheduled-downtime-due-to-data-center-power-test-on-september-15/18384)
**Author:** [@team-freiburg](https://help.galaxyproject.org/u/team-freiburg)\ **Replies:** 0\ **Last updated:** [September 14, 2026, 9:42am UTC](https://help.galaxyproject.org/t/scheduled-downtime-due-to-data-center-power-test-on-september-15/18384 "2026-09-14T09:42:11Z")
Due to a scheduled data center power cut and recovery test, there will be a downtime on 15th September, from 7:30 a.m. to at least 10:30 a.m. CEST. This test will improve our data center’s resilience against power outage… --- ## [Failed to betaupload](https://help.galaxyproject.org/t/failed-to-betaupload/18377)
**Author:** [@arbv](https://help.galaxyproject.org/u/arbv)\ **Replies:** 1\ **Last updated:** [September 11, 2026, 8:06pm UTC](https://help.galaxyproject.org/t/failed-to-betaupload/18377 "2026-09-11T20:06:35Z")
Server: usegalaxy.org Upload method: Import Data (Beta) File: MDMS240000022-3a\_L1\_1.fq.gz Size: 6.1 GB Target history: SJ29\_assembly\_mapping\_BWA Upload Progress shows 100% completed, but the dataset does not appear … --- ## [Requesting Activation of FTP](https://help.galaxyproject.org/t/requesting-activation-of-ftp/18379)
**Author:** [@ephillippi](https://help.galaxyproject.org/u/ephillippi)\ **Replies:** 1\ **Last updated:** [September 10, 2026, 6:22pm UTC](https://help.galaxyproject.org/t/requesting-activation-of-ftp/18379 "2026-09-10T18:22:13Z")
I am trying to upload 100-300GB files for analysis. The upload has failed multiple times in the web uploader. I really need to be able to restart an upload when there are network hiccups, but FTP is not an option availab… --- ## [Data Upload delays UseGalaxy.org Sept 2026](https://help.galaxyproject.org/t/data-upload-delays-usegalaxy-org-sept-2026/18375)
**Author:** [@Linda\_Kaira](https://help.galaxyproject.org/u/Linda_Kaira)\ **Replies:** 3\ **Last updated:** [September 10, 2026, 4:45pm UTC](https://help.galaxyproject.org/t/data-upload-delays-usegalaxy-org-sept-2026/18375 "2026-09-10T16:45:50Z")
Good day, I have an issue of my data having queued for over 24 hours and even if I upload new data I am still receiving a queued status. I have even started a new history but it is still giving me a queued status --- ## [How to run VGP workflow with large files?](https://help.galaxyproject.org/t/how-to-run-vgp-workflow-with-large-files/18368)
**Author:** [@jinglin](https://help.galaxyproject.org/u/jinglin)\ **Replies:** 2\ **Last updated:** [September 9, 2026, 6:30am UTC](https://help.galaxyproject.org/t/how-to-run-vgp-workflow-with-large-files/18368 "2026-09-09T06:30:20Z")
I’m trying to run VGP workflow for my genome assembly, but my HiFi data is large which is 230GB. It is about 60GB in compressed (.gz) format, but VGP workflow only accept uncompressed files. My total storage size is only… --- ## [Jobs queues UseGalaxy.eu August 2026](https://help.galaxyproject.org/t/jobs-queues-usegalaxy-eu-august-2026/18289)
**Author:** [@JGojznikar](https://help.galaxyproject.org/u/JGojznikar)\ **Replies:** 6\ **Last updated:** [September 7, 2026, 9:53pm UTC](https://help.galaxyproject.org/t/jobs-queues-usegalaxy-eu-august-2026/18289 "2026-09-07T21:53:50Z")
Hello everyone, I’ve currently scheduled a test workflow run on a subset of samples. The workflow passed previous runs without any clear issue, however now has been on scheduled (“pending”) for more than two weeks. On a… --- ## [Prodigal Gene Predictor: error option (# Other genetic codes 1-25) stderr Invalid translation table specified](https://help.galaxyproject.org/t/prodigal-gene-predictor-error-option-other-genetic-codes-1-25-stderr-invalid-translation-table-specified/18350)
**Author:** [@tvtv195](https://help.galaxyproject.org/u/tvtv195)\ **Replies:** 1\ **Last updated:** [September 7, 2026, 5:55pm UTC](https://help.galaxyproject.org/t/prodigal-gene-predictor-error-option-other-genetic-codes-1-25-stderr-invalid-translation-table-specified/18350 "2026-09-07T17:55:12Z")
I am experiencing a bug in this specific version of the Galaxy wrapper (Galaxy Version 2.6.3+galaxy0). The developer forgot to link the conditional text field container to the # : Other genetic codes 1-25 selection choi… --- ## [STARsolo custom reference fails during genomeGenerate — need genomeChrBinNbits for fragmented Nicotiana genome](https://help.galaxyproject.org/t/starsolo-custom-reference-fails-during-genomegenerate-need-genomechrbinnbits-for-fragmented-nicotiana-genome/18360)
**Author:** [@fforn](https://help.galaxyproject.org/u/fforn)\ **Replies:** 4\ **Last updated:** [September 3, 2026, 6:53pm UTC](https://help.galaxyproject.org/t/starsolo-custom-reference-fails-during-genomegenerate-need-genomechrbinnbits-for-fragmented-nicotiana-genome/18360 "2026-09-03T18:53:22Z")
Hi Galaxy Europe team, I am trying to reconstruct a published 10x Genomics v3 single-nucleus RNA-seq dataset from Nicotiana tabacum K326 using RNA STARsolo on singlecell.usegalaxy.eu. Tool: RNA STARsolo 2.7.11b+galaxy2 … --- ## [Dorado now missing from usegalaxy.eu site...?](https://help.galaxyproject.org/t/dorado-now-missing-from-usegalaxy-eu-site/18352)
**Author:** [@scyllas1981](https://help.galaxyproject.org/u/scyllas1981)\ **Replies:** 4\ **Last updated:** [September 3, 2026, 1:55am UTC](https://help.galaxyproject.org/t/dorado-now-missing-from-usegalaxy-eu-site/18352 "2026-09-03T01:55:48Z")
Hi all - hoping an admin usegalaxy.e.u could help Today I can’t seem to find dorado in the tools - has it been removed??? Have used recently, this seems a significant change if removed. Thanks Graham --- ## [Galaxy IT Physicell: execution troubleshooting](https://help.galaxyproject.org/t/galaxy-it-physicell-execution-troubleshooting/18367)
**Author:** [@rheiland](https://help.galaxyproject.org/u/rheiland)\ **Replies:** 2\ **Last updated:** [September 2, 2026, 6:12pm UTC](https://help.galaxyproject.org/t/galaxy-it-physicell-execution-troubleshooting/18367 "2026-09-02T18:12:43Z")
I seem to have a similar problem - my IT will not start. Here’s my shared History: Galaxy --- ## [File remain greyed out](https://help.galaxyproject.org/t/file-remain-greyed-out/16505)
**Author:** [@Nadya](https://help.galaxyproject.org/u/Nadya)\ **Replies:** 1\ **Last updated:** [November 19, 2025, 9:51pm UTC](https://help.galaxyproject.org/t/file-remain-greyed-out/16505 "2025-11-19T21:51:00Z")
Hello, Galaxy was working fine for me for several hours, but after I uploaded another relatively small file, it has remained greyed out for hours. Is there anything I can do to get it working again, or are there any iss… --- ## [How to structure design file for Deseq2 analysis on multiple factors](https://help.galaxyproject.org/t/how-to-structure-design-file-for-deseq2-analysis-on-multiple-factors/18353)
**Author:** [@Nadia2112](https://help.galaxyproject.org/u/Nadia2112)\ **Replies:** 1\ **Last updated:** [September 2, 2026, 7:01am UTC](https://help.galaxyproject.org/t/how-to-structure-design-file-for-deseq2-analysis-on-multiple-factors/18353 "2026-09-02T07:01:45Z")
After running KallistoQuant, I need to analyze my data on DeSeq2. The factors I have in my dataset are genotype (2 genotypes, WT and MUT) and treatment (3 treatments, R, L and V) and I would like to analyze them both sep… --- ## [IGV visualization fail](https://help.galaxyproject.org/t/igv-visualization-fail/18354)
**Author:** [@scholtz](https://help.galaxyproject.org/u/scholtz)\ **Replies:** 1\ **Last updated:** [August 31, 2026, 4:10pm UTC](https://help.galaxyproject.org/t/igv-visualization-fail/18354 "2026-08-31T16:10:52Z")
I tried to visualize my aligned files (merged bam, generated with RNA STAR) directly from Galaxy.eu with local IGV, but received the following warning from IGV: Error message: Unknown BAM index file type: null in null. N… --- ## [Gromacs exceeded 60h](https://help.galaxyproject.org/t/gromacs-exceeded-60h/18363)
**Author:** [@Turki](https://help.galaxyproject.org/u/Turki)\ **Replies:** 0\ **Last updated:** [August 31, 2026, 3:22pm UTC](https://help.galaxyproject.org/t/gromacs-exceeded-60h/18363 "2026-08-31T15:22:22Z")
Hello, I have a GROMACS 2022+galaxy0 molecular dynamics job on usegalaxy.eu using GPU resources. The job has been in the running state for approximately 60 hours. Standard output and standard error are empty, and the int… --- ## [Reading old support threads — do you prefer follow-ups as new topics?](https://help.galaxyproject.org/t/reading-old-support-threads-do-you-prefer-follow-ups-as-new-topics/18335)
**Author:** [@roadtrip6587](https://help.galaxyproject.org/u/roadtrip6587)\ **Replies:** 3\ **Last updated:** [August 29, 2026, 1:36pm UTC](https://help.galaxyproject.org/t/reading-old-support-threads-do-you-prefer-follow-ups-as-new-topics/18335 "2026-08-29T13:36:18Z")
Hi everyone, relatively new here. I’ve been going through older threads while learning the platform and often find half-answered questions. Quick question about local habits: when an old topic is close to what I want to… --- ## [Pre.cluster (mothur) empty outputs: consider using groups! Please see tutorial example](https://help.galaxyproject.org/t/pre-cluster-mothur-empty-outputs-consider-using-groups-please-see-tutorial-example/18347)
**Author:** [@Heba\_Hathout](https://help.galaxyproject.org/u/Heba_Hathout)\ **Replies:** 1\ **Last updated:** [August 27, 2026, 4:27pm UTC](https://help.galaxyproject.org/t/pre-cluster-mothur-empty-outputs-consider-using-groups-please-see-tutorial-example/18347 "2026-08-27T16:27:53Z")
Server URL: usegalaxy dot org I’m running the Pre.cluster tool (mothur, Galaxy Version 1.39.5.0) on a 16S mothur pipeline (23 samples, RedSeaSeawater\_16S\_Mothur pipeline history). The job has been stuck in “running” s… --- ## [NOVOplasty new version](https://help.galaxyproject.org/t/novoplasty-new-version/18314)
**Author:** [@M\_gras](https://help.galaxyproject.org/u/M_gras)\ **Replies:** 4\ **Last updated:** [August 26, 2026, 10:27am UTC](https://help.galaxyproject.org/t/novoplasty-new-version/18314 "2026-08-26T10:27:50Z")
Hello, I use NOVOplasty for mitogenome assembly. For one assembly I need the option " Reduce ambiguous N’s " and it’s available with the version 4.3.3 Is it possible to add the version please ??? Thank you very much M… --- ## [Jobs stuck in 'new' state — Faster Download and Extract Reads in FASTQ](https://help.galaxyproject.org/t/jobs-stuck-in-new-state-faster-download-and-extract-reads-in-fastq/18337)
**Author:** [@Ghaida](https://help.galaxyproject.org/u/Ghaida)\ **Replies:** 4\ **Last updated:** [August 25, 2026, 12:12pm UTC](https://help.galaxyproject.org/t/jobs-stuck-in-new-state-faster-download-and-extract-reads-in-fastq/18337 "2026-08-25T12:12:55Z")
Resolved :white\_check\_mark: Tool name: “Faster Download and Extract Reads in FASTQ format from NCBI SRA (Galaxy Version 3.1.1+galaxy1)” Clicking Run Tool shows ‘please wait’ for under a second, then reverts with no job… --- ## [RStudio interactive tool doesn't load, seemingly because of http/https mix](https://help.galaxyproject.org/t/rstudio-interactive-tool-doesnt-load-seemingly-because-of-http-https-mix/18294)
**Author:** [@Peter\_van\_Heusden](https://help.galaxyproject.org/u/Peter_van_Heusden)\ **Replies:** 2\ **Last updated:** [August 25, 2026, 11:47am UTC](https://help.galaxyproject.org/t/rstudio-interactive-tool-doesnt-load-seemingly-because-of-http-https-mix/18294 "2026-08-25T11:47:48Z")
Hi there When I try and get a RStudio session I get a blank page that seems to result from the interactive tool being linked to via http instead of https: Loading the url with https works. Is this a config error on … --- ## [Request to update the Kraken2 microsporidia database (EuPathDB-46)](https://help.galaxyproject.org/t/request-to-update-the-kraken2-microsporidia-database-eupathdb-46/18341)
**Author:** [@Sing-P](https://help.galaxyproject.org/u/Sing-P)\ **Replies:** 1\ **Last updated:** [August 25, 2026, 3:34am UTC](https://help.galaxyproject.org/t/request-to-update-the-kraken2-microsporidia-database-eupathdb-46/18341 "2026-08-25T03:34:55Z")
I’m currently working on microsporidia genome analysis, and Kraken2 is an essential tool in my workflow. The microsporidia-specific Kraken2 database currently available is EuPathDB-46 (2023 version). Over the past 2–3 ye… --- ## [Immediate error when running RNA STAR](https://help.galaxyproject.org/t/immediate-error-when-running-rna-star/18319)
**Author:** [@knmcfarland](https://help.galaxyproject.org/u/knmcfarland)\ **Replies:** 2\ **Last updated:** [August 25, 2026, 3:31am UTC](https://help.galaxyproject.org/t/immediate-error-when-running-rna-star/18319 "2026-08-25T03:31:02Z")
Hi! I’m trying to align fastq files to the built in mouse genome (GRCm39/mm39) using RNA STAR. I am getting errors of “Encountered an unhandled exception while caching job destination dynamic rule” almost immediately or … --- ## [Usegalaxy.eu HUMAnN 3.9+galaxy1 fails because ChocoPhlAn directory contains mixed database versions](https://help.galaxyproject.org/t/usegalaxy-eu-humann-3-9-galaxy1-fails-because-chocophlan-directory-contains-mixed-database-versions/18339)
**Author:** [@Hc\_G](https://help.galaxyproject.org/u/Hc_G)\ **Replies:** 1\ **Last updated:** [August 25, 2026, 3:29am UTC](https://help.galaxyproject.org/t/usegalaxy-eu-humann-3-9-galaxy1-fails-because-chocophlan-directory-contains-mixed-database-versions/18339 "2026-08-25T03:29:16Z")
Hello Galaxy team, I am running HUMAnN on usegalaxy.eu and encountered the same ChocoPhlAn version error reported in this previous topic: https://help.galaxyproject.org/t/humann-fails-due-to-metaphlan-database-mismatch… [Previous page](https://help.galaxyproject.org/latest.md?page=1) [Next page](https://help.galaxyproject.org/latest.md?page=3)