# Add bacterial genes to a plant genome file

**URL:** <https://help.galaxyproject.org/t/add-bacterial-genes-to-a-plant-genome-file/9939>\
**Category:** Uncategorized\
**Tags:** data-manipulation, transcriptomics\
**Created:** [April 21, 2023, 4:05pm UTC](https://help.galaxyproject.org/t/add-bacterial-genes-to-a-plant-genome-file/9939 "2023-04-21T16:05:36Z")\
**Posts on this page:** 1\
**Showing post:** 2

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [April 21, 2023, 6:09pm UTC](https://help.galaxyproject.org/t/add-bacterial-genes-to-a-plant-genome-file/9939/2 "2023-04-21T18:09:47Z")

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Hi @brebelo

This recent Q&A explains what is needed: [RNA seq analysis on transgenic animal](https://help.galaxyproject.org/t/rna-seq-analysis-on-transgenic-animal/9742)

In short, combine the fasta for the two different organisms, then add in some lines to the existing reference annotation to represent the extra genes/transgenes. This will take some trial and error to get right.

Working with a 4 GB file is fine when doing data manipulations in Galaxy.

> **[Galaxy Training: Data Manipulation Olympics](https://training.galaxyproject.org/training-material/topics/introduction/tutorials/data-manipulation-olympics/tutorial.html)**
>
> Galaxy is a scientific workflow, data integration, and da...

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