# AlphaFold2 Error - Could not find HHsearch database /data/pdb70/pdb70 **URL:** \ **Category:** usegalaxy.eu support\ **Tags:** proteomics\ **Created:** [September 2, 2022, 5:20pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593 "2022-09-02T17:20:55Z")\ **Posts on this page:** 11\ **Page:** 1
**Author:** ![sunag\_parasu](https://avatars.discourse-cdn.com/v4/letter/s/aeb1de/32.png) [@sunag\_parasu](https://help.galaxyproject.org/u/sunag_parasu)\ **Post date:** [September 2, 2022, 5:20pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/1 "2022-09-02T17:20:55Z")
I tried running a FASTA sequence on AlphaFold2 and it showed me this error: E0902 16:02:23.065268 22797118093120 hhsearch.py:56] Could not find HHsearch database /data/pdb70/pdb70 Traceback (most recent call last): File “/app/alphafold/run\_alphafold.py”, line 445, in app.run(main) File “/opt/conda/lib/python3.7/site-packages/absl/app.py”, line 312, in run \_run\_main(main, args) File “/opt/conda/lib/python3.7/site-packages/absl/app.py”, line 258, in \_run\_main sys.exit(main(argv)) File “/app/alphafold/run\_alphafold.py”, line 353, in main databases=[FLAGS.pdb70\_database\_path]) File “/app/alphafold/alphafold/data/tools/hhsearch.py”, line 57, in **init** raise ValueError(f’Could not find HHsearch database {database\_path}') ValueError: Could not find HHsearch database /data/pdb70/pdb70 This is the sequence I tried to run: > 33 > MPAPAPLLIILRRRIRKQAHAHSKGGGGSMR > TSYLLLFTLCLLLSEMASGGNFLTGLGHRSD > HYNCVSSGGQCLYSACPIFTKIQGTCYRGKA > KCCKEAAAKFPGVGFPGVGFPGVGKPKPKPL > GIYVYVRRKKSLSGCYWCLKKFSWWDPGFFI > KKGLPIRASRLVFKKKRRCAVLLYKKVIFSN > ISNWVYKKRLGNGTFYKKLQVQFAVFKKNSP > YIPYLLKKSFQAWLTNQPYKKCAQLRYLVFK > KFIAVIPAANFKKYAIALVAELLKKMNITGY > TDAYKKLLLAITSNIVKKLIKGNFLEYVKKS > LSGCYWCLIKKIYVYVRRCVLKKFAVFGILP > CVKKDSQFTLIKPYKKPLGLWARVLAEALII > GFSWWDPGFFIIAAYSFQAWLTNQPYASFMV > AAYFIAVIPAANFPGVLLTQAAYSVIFSNIS > NWVYAAYTVYFLPNSIITNVFFIAAYMVSLI > YLASFLFKSSSTAAAYRLISTALKARALSLD > SAAYRRRLANSLRLATVIYGKAAYEIRWSFT > LNCSASDSEAAYEYVRILMRKLLLQITRHHH > HHH Please let me know if there is an error with my input or within the tool Thank you ---
**Author:** ![gallardoalba](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/gallardoalba/32/1903_2.png) [@gallardoalba](https://help.galaxyproject.org/u/gallardoalba)\ **Post date:** [September 5, 2022, 9:46am UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/2 "2022-09-05T09:46:24Z")
Hi @sunag_parasu, the problem is caused by the tool itself. I suggest you to try to run your analysis in the Australian Galaxy server; you can apply in the following link: [AlphaFold 2.0](https://site.usegalaxy.org.au/request/alphafold). Regards ---
**Author:** ![Ulises\_Mancini](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/ulises_mancini/32/3725_2.png) [@Ulises\_Mancini](https://help.galaxyproject.org/u/Ulises_Mancini)\ **Post date:** [October 11, 2022, 5:32pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/3 "2022-10-11T17:32:36Z")
The problem is that if we don’t have an email from an Australian institution, we can’t apply to that server. Any other option? ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [October 11, 2022, 6:38pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/4 "2022-10-11T18:38:22Z")
Hi @Ulises_Mancini The AU server is the only public Galaxy server option that I know of. ---
**Author:** ![Ulises\_Mancini](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/ulises_mancini/32/3725_2.png) [@Ulises\_Mancini](https://help.galaxyproject.org/u/Ulises_Mancini)\ **Post date:** [October 11, 2022, 6:52pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/5 "2022-10-11T18:52:13Z")
Hi Jenn To access the Alphafold 2.0 tool on the Australian server, you need to register with an email from an Australian institution. Search in google “Galaxy Australia AlphaFold 2.0 Access Request” Best! ---
**Author:** ![bjoern.gruening](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/bjoern.gruening/32/10_2.png) [@bjoern.gruening](https://help.galaxyproject.org/u/bjoern.gruening)\ **Post date:** [March 18, 2023, 8:10pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/6 "2023-03-18T20:10:32Z")
Hi, we have update Alphafold2 on the EU server to the latest version and also the underlying database. Please try to new version 2.3.1 ([Galaxy | Europe](https://usegalaxy.eu/root?tool_id=toolshed.g2.bx.psu.edu/repos/galaxy-australia/alphafold2/alphafold/2.3.1+galaxy0)) Happy research! Bjoern ---
**Author:** ![Julian\_Martinez\_Maye](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/julian_martinez_maye/32/4272_2.png) [@Julian\_Martinez\_Maye](https://help.galaxyproject.org/u/Julian_Martinez_Maye)\ **Post date:** [May 9, 2023, 4:14pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/7 "2023-05-09T16:14:14Z")
Hi, I’m getting the exact same error. Any way to solve this? Thank you ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [May 9, 2023, 4:32pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/8 "2023-05-09T16:32:35Z")
Welcome, @Julian_Martinez_Maye Current `Alphafold` availability is… - **[UseGalaxy.eu](http://UseGalaxy.eu)** → supports everyone - **[UseGalaxy.org.au](http://UseGalaxy.org.au)** → supports AU academic users - **[UseGalaxy.org](http://UseGalaxy.org)** → not supported at all for now Did the error come up at [UseGalaxy.eu](http://UseGalaxy.eu)? If you haven’t tried there yet, please do and let us know how that goes 🙂 ---
**Author:** ![Julian\_Martinez\_Maye](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/julian_martinez_maye/32/4272_2.png) [@Julian\_Martinez\_Maye](https://help.galaxyproject.org/u/Julian_Martinez_Maye)\ **Post date:** [May 9, 2023, 4:44pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/9 "2023-05-09T16:44:36Z")
Thanks for the quick answer. Yes, the problem seems to come up in the **[UseGalaxy.eu](http://usegalaxy.eu/)** page. Best, Julián ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [May 9, 2023, 5:19pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/10 "2023-05-09T17:19:59Z")
Ok, thanks for clarifying. Please submit a bug report from that red error dataset to report the problem to the EU administrators. You can include a link to this topic in the comments for context. cc @bjoern.gruening @gallardoalba ---
**Author:** ![jdom](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jdom/32/4460_2.png) [@jdom](https://help.galaxyproject.org/u/jdom)\ **Post date:** [May 12, 2023, 1:35pm UTC](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/11 "2023-05-12T13:35:57Z")
As of today, this problem should be solved on [usegalaxy.eu](http://usegalaxy.eu/), at least for the latest version of the tool (2.3.1+galaxy2). We are still running some more test cases for the latest version as well as for the older ones to make sure that this error is not arising in older versions of the tool.