# Alphafold2 Error Message - Could not find HHsearch database /data/pdb70/pdb70

**URL:** <https://help.galaxyproject.org/t/alphafold2-error-message-could-not-find-hhsearch-database-data-pdb70-pdb70/8163>\
**Category:** usegalaxy.org support\
**Tags:** proteomics\
**Created:** [June 7, 2022, 9:40am UTC](https://help.galaxyproject.org/t/alphafold2-error-message-could-not-find-hhsearch-database-data-pdb70-pdb70/8163 "2022-06-07T09:40:04Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![hongkarwai](https://avatars.discourse-cdn.com/v4/letter/h/e19b73/32.png) [@hongkarwai](https://help.galaxyproject.org/u/hongkarwai)\
**Post date:** [June 7, 2022, 9:40am UTC](https://help.galaxyproject.org/t/alphafold2-error-message-could-not-find-hhsearch-database-data-pdb70-pdb70/8163/1 "2022-06-07T09:40:04Z")

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I was running a collection of FASTA files on ALphafold2. Each FASTA file contains a single line of FASTA header and a single line of amino acid sequence. The run failed and the error message generated was “Could not find HHsearch database /data/pdb70/pdb70”.

 ![Screenshot from 2022-06-07 17:36:43](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/b/b37dc127b45548d4529ba800651fed1b759de277.png)  
 ![Screenshot from 2022-06-07 17:35:28](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/e/e8f359788b73c28ee98111e4781d83116102d752.png)

What should I do now so that I can run the structure prediction?

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [September 21, 2022, 9:45pm UTC](https://help.galaxyproject.org/t/alphafold2-error-message-could-not-find-hhsearch-database-data-pdb70-pdb70/8163/2 "2022-09-21T21:45:02Z")

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Known tool issue. See [AlphaFold2 Error - Could not find HHsearch database /data/pdb70/pdb70 - #2 by gallardoalba](https://help.galaxyproject.org/t/alphafold2-error-could-not-find-hhsearch-database-data-pdb70-pdb70/8593/2)

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [September 21, 2022, 9:45pm UTC](https://help.galaxyproject.org/t/alphafold2-error-message-could-not-find-hhsearch-database-data-pdb70-pdb70/8163/3 "2022-09-21T21:45:10Z")

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