# An error of Trinity de novo assembly of RNA-seq data -- Disabled tool, use RNA-Spades instead **URL:** \ **Category:** usegalaxy.org support\ **Tags:** assembly, transcriptomics, tool-deprecated, fastqsanger, rnaspades\ **Created:** [June 21, 2022, 7:53pm UTC](https://help.galaxyproject.org/t/an-error-of-trinity-de-novo-assembly-of-rna-seq-data-disabled-tool-use-rna-spades-instead/8229 "2022-06-21T19:53:13Z")\ **Posts on this page:** 6\ **Page:** 1
**Author:** ![tiredpenguinD](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/tiredpenguind/32/3421_2.png) [@tiredpenguinD](https://help.galaxyproject.org/u/tiredpenguinD)\ **Post date:** [June 21, 2022, 7:53pm UTC](https://help.galaxyproject.org/t/an-error-of-trinity-de-novo-assembly-of-rna-seq-data-disabled-tool-use-rna-spades-instead/8229/1 "2022-06-21T19:53:13Z")
Hi I tried run Trinity on server of [Galaxy.org](http://Galaxy.org) and it suddenly finished with error message below. “This tools currently disabled, please see Galaxy Help site at [help.galaxyproject.org](http://help.galaxyproject.org) for assistance.” And to see the detail of error, I opened the window. ![Screenshot from 2022-06-21 15-48-59](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/d/d29493e0f0bcb924c1665f84f2b8cd52ccef2f7b.png) An error occurred while running the tool **toolshed.g2.bx.psu.edu/repos/iuc/trinity/trinity/2.9.1+galaxy2**. The used file type was fastqsanger.gz and the tetrameter was like the second picture. ![Screenshot from 2022-06-21 15-51-34](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/6/65f3e2b5c9be5affd671a06fbd78d7acb61cb748.png) Do anyone have a solution for this problem? Thanks ---
**Author:** ![Pedro\_Luis\_Ramos-Gon](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/pedro_luis_ramos-gon/32/3449_2.png) [@Pedro\_Luis\_Ramos-Gon](https://help.galaxyproject.org/u/Pedro_Luis_Ramos-Gon)\ **Post date:** [June 21, 2022, 8:19pm UTC](https://help.galaxyproject.org/t/an-error-of-trinity-de-novo-assembly-of-rna-seq-data-disabled-tool-use-rna-spades-instead/8229/2 "2022-06-21T20:19:45Z")
During the last ten days, I have also experienced the same issue. ---
**Author:** ![gallardoalba](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/gallardoalba/32/1903_2.png) [@gallardoalba](https://help.galaxyproject.org/u/gallardoalba)\ **Post date:** [June 22, 2022, 9:05am UTC](https://help.galaxyproject.org/t/an-error-of-trinity-de-novo-assembly-of-rna-seq-data-disabled-tool-use-rna-spades-instead/8229/3 "2022-06-22T09:05:12Z")
Hi @Pedro_Luis_Ramos-Gon and @tiredpenguinD, I’ll ask the system admins about that. Thanks for the report! ---
**Author:** ![gallardoalba](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/gallardoalba/32/1903_2.png) [@gallardoalba](https://help.galaxyproject.org/u/gallardoalba)\ **Post date:** [June 22, 2022, 9:14am UTC](https://help.galaxyproject.org/t/an-error-of-trinity-de-novo-assembly-of-rna-seq-data-disabled-tool-use-rna-spades-instead/8229/4 "2022-06-22T09:14:10Z")
We are experiencing some memory issues with Trinity; until the problem is corrected, I suggest you to use rnaSPAdes. Regards ---
**Author:** ![tiredpenguinD](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/tiredpenguind/32/3421_2.png) [@tiredpenguinD](https://help.galaxyproject.org/u/tiredpenguinD)\ **Post date:** [June 22, 2022, 7:14pm UTC](https://help.galaxyproject.org/t/an-error-of-trinity-de-novo-assembly-of-rna-seq-data-disabled-tool-use-rna-spades-instead/8229/5 "2022-06-22T19:14:31Z")
Hi @gallardoalba , Thank you for your advice! I’ll try to use rnaSPAdes. Thanks ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [July 23, 2022, 12:50am UTC](https://help.galaxyproject.org/t/an-error-of-trinity-de-novo-assembly-of-rna-seq-data-disabled-tool-use-rna-spades-instead/8229/6 "2022-07-23T00:50:42Z")