I have tried to annotate my geneID con this app but an I don’t get success. I have inputted the count table from featureCounts with Ensembl human nomenclature e.g ENST00000000233.10
Execution resulted in the following messages:
Fatal error: Exit code 1 ()
I have tried either Ensembl gene and Ensembl transcript and It didn’t work.
These are Ensembl transcript identifiers, not genes.
Choices:
Accept that the counts will be “by transcript” and not “by gene” and remove the .NN where N is the version number after the dot.
Choose a different reference annotation GTF dataset where the gene_id attribute is actually a gene. Some data providers host GTFs where the transcript_id and gene_id are the same within a GTF dataset.
Use Featurecounts for generating counts and use the built-in annotation (Entrez).
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