# BUSCO error: refseq db not found

**URL:** <https://help.galaxyproject.org/t/busco-error-refseq-db-not-found/16328>\
**Category:** usegalaxy.org support\
**Tags:** genome-annotation, resources, tool-help, busco\
**Created:** [October 1, 2025, 8:21am UTC](https://help.galaxyproject.org/t/busco-error-refseq-db-not-found/16328 "2025-10-01T08:21:12Z")\
**Posts on this page:** 1\
**Showing post:** 6

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [October 3, 2025, 5:48pm UTC](https://help.galaxyproject.org/t/busco-error-refseq-db-not-found/16328/6 "2025-10-03T17:48:51Z")

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Hi @H1889

I was just setting the default combinations at the top level to show what would be produced – my example isn’t a scientific example, more about the settings. I should have worded this better! I’ll try again.

You have bacterial data, correct? Using **Metaeuk** is the only requirement, the remainder you can set as needed. That can be “auto” for the linage as I used – but you can also choose a linage. In short, using **Miniprot** won’t work with the “auto” choice because it only functions with a linage selection.

Some more details are here. → [https://help.galaxyproject.org/t/busco-for-bacterial-genome-assemblies/14475](https://help.galaxyproject.org/t/busco-for-bacterial-genome-assemblies/14475)

Does this now help? 🙂

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