# BUSCO for bacterial genome assemblies **URL:** \ **Category:** usegalaxy.org support\ **Tags:** tool-help, busco\ **Created:** [January 17, 2025, 10:51am UTC](https://help.galaxyproject.org/t/busco-for-bacterial-genome-assemblies/14475 "2025-01-17T10:51:13Z")\ **Posts on this page:** 2\ **Page:** 1
**Author:** ![Touraj\_Rahimi](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/touraj_rahimi/32/6633_2.png) [@Touraj\_Rahimi](https://help.galaxyproject.org/u/Touraj_Rahimi)\ **Post date:** [January 17, 2025, 10:51am UTC](https://help.galaxyproject.org/t/busco-for-bacterial-genome-assemblies/14475/1 "2025-01-17T10:51:13Z")
Hi, I’m trying to run BUSCO for some bacterial genome assemblies, in this case prodigal use as default gene prediction, but in galaxy eu, choosing one of three **Select a gene predictor** options which uses mainly for eukaryotic data has signifcant effect on BUSCO results in bacterial analysis. Any advice how to use it for bacterial genome? Best ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [January 17, 2025, 5:40pm UTC](https://help.galaxyproject.org/t/busco-for-bacterial-genome-assemblies/14475/2 "2025-01-17T17:40:37Z")
Welcome, @Touraj_Rahimi We have some advice in this topic. Maybe try using **MetaEuk**? > [@BUSCO Fatal error: Exit code 1 ()](https://help.galaxyproject.org/t/busco-fatal-error-exit-code-1/13544/5): > > The key problem is that I was not leaving selected MetaEuk as database, as I was working with prokaryotes, but I realized now that the code works with Prodigal in bacterial lineages even if there is no option to select it. I was then selecting Miniprot and I was getting the error. So to solve it it was only a matter of leaving MetaEuk selected for prokaryon genomes. You could also try **Prokka** or **Funannotate** for annotation. Let’s start there, thanks! 🙂