Cannot get QIIME2 artifact to work

Welcome, @mbyec18

The first step with the Qiime tools is to get your data into an artifact. There are a few types, and there isn’t a lot of Galaxy specific documentation for this – just use the original documentation from the tool authors. Your current errors about finding the “wrong” artifact type.

Then the next step, is about if you are having trouble (this is where you are now), is to loop back to examine your artifact type/content against the type of artifact that the downstream tools is expecting to work with.

There is a web site the authors host where you can review the current artifact content, and their documentation explains what you will need instead. Then you’ll need to create that and try again. This can be a bit technical so start at the very first tutorial the authors host, and maybe visit their forum, since they know this software best. All qza artifacts are like a little database: files and metadata. What kind of data, and how it is organized, can vary a great deal!

We do have some prior QA here. One is where I walked through and created an artifact for someone, and the others have related discussions. Search results for 'qiime order:latest' - Galaxy Community Help

So, in short: get the right artifact created for your target tool done first to avoid this type of error. Then you can focus on that tool’s usage in more detail (data, parameters). Right now all of your errors are technical message about the formats (and not even the data format yet, instead the artifact format).

Or, you can back up and determine why the same tools, used outside of the Qiime package, are failing and address that instead. These will be regular files, and not nested into their own “database” the same way the artifacts are, so are a bit easier to work with, and more transparently.

Both Mothur and DADA2 have tutorials linked on the bottom of the tool forms. And if you have a specific error with those, we are much more likely to be able to help.

Thanks, and hope this helps to understand what is going on! :slight_smile:

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