# Comparing two different RNAseq datasets

**URL:** <https://help.galaxyproject.org/t/comparing-two-different-rnaseq-datasets/1206>\
**Category:** Uncategorized\
**Created:** [April 29, 2019, 12:48pm UTC](https://help.galaxyproject.org/t/comparing-two-different-rnaseq-datasets/1206 "2019-04-29T12:48:15Z")\
**Posts on this page:** 1\
**Showing post:** 2

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [May 3, 2019, 7:26pm UTC](https://help.galaxyproject.org/t/comparing-two-different-rnaseq-datasets/1206/2 "2019-05-03T19:26:48Z")

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Hi @Ubaid_Midhat!

The `Deeptools` suite might be what you are looking for to get information about the sequence depth/coverage variation between your samples. Please see: [https://deeptools.readthedocs.io/](https://deeptools.readthedocs.io/)

There is a domain-specific Galaxy server hosted through that web site, but you’ll also find these tools at public Galaxy servers. Examples: Galaxy EU [https://usegalaxy.eu](https://usegalaxy.eu) and Galaxy Main [https://usegalaxy.org](https://usegalaxy.org).

I’m not sure what your analysis goals are (expression? variation?) but the Galaxy tutorials might be helpful. Many tools have normalization functions/options. Please see:

> [@Troubleshooting resources for errors or unexpected results](https://help.galaxyproject.org/t/troubleshooting-resources-for-errors-or-unexpected-results/42/1):
>
> [Galaxy Training Network Tutorials](https://galaxyproject.github.io/training-material/): Some [GTN](https://galaxyproject.org/teach/gtn/) tutorials are appropriate for [Galaxy Main](https://usegalaxy.org) and some are not. Where you can run each is noted per tutorial – click on the _Galaxy instances_ gear icon ![galaxyserversGTN](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/1X/1287f50cfcdaa1aad750fe323f96995260ac4947.png) to review the [Public Galaxy](https://galaxyproject.org/use/) server choices. If a tutorial is supported by a pre-configured [Galaxy Docker](https://hub.docker.com/r/bgruening/galaxy-stable/) training image, instructions for how to get it will be listed below the tutorial listings, per category.

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_[View the full topic](https://help.galaxyproject.org/t/comparing-two-different-rnaseq-datasets/1206)._