# Convert RNA STARsolo output to AnnData format

**URL:** <https://help.galaxyproject.org/t/convert-rna-starsolo-output-to-anndata-format/12227>\
**Category:** usegalaxy.org support\
**Tags:** single-cell\
**Created:** [April 16, 2024, 10:58pm UTC](https://help.galaxyproject.org/t/convert-rna-starsolo-output-to-anndata-format/12227 "2024-04-16T22:58:41Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Priyanka\_Bhandary](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/priyanka_bhandary/32/4150_2.png) [@Priyanka\_Bhandary](https://help.galaxyproject.org/u/Priyanka_Bhandary)\
**Post date:** [April 16, 2024, 10:58pm UTC](https://help.galaxyproject.org/t/convert-rna-starsolo-output-to-anndata-format/12227/1 "2024-04-16T22:58:41Z")

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Hello,

Thank you for creating this community for Galaxy questions. I have been designing a workflow for single-cell RNA-seq analysis. I am using the RNA STARsolo tool to carry out demultiplexing and quantification. It gives me output in tsv (Barcodes and genes) and mtx (counts) format. I referred to this tutorial for converting different scRNA-seq data formats - [Hands-on: Converting between common single cell data formats / Single Cell](https://training.galaxyproject.org/training-material/topics/single-cell/tutorials/scrna-data-ingest/tutorial.html). I went through it and couldn’t find any tool that could convert the tsv and mtx format to AnnData format. I need the AnnData format to run the downstream analysis using ScanPy. I combed through the tools in Galaxy but couldn’t find any tools to do this conversion. Are there any tools that can do this? Thank you!

Priyanka

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [April 18, 2024, 7:29pm UTC](https://help.galaxyproject.org/t/convert-rna-starsolo-output-to-anndata-format/12227/2 "2024-04-18T19:29:34Z")

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Hi @Priyanka_Bhandary

Would this tool do what you need? It has a tutorial and extra help down in the Help section on the tool form for more context → **Import Anndata and loom** from different format