Demultiplexing and Quantification of 10X Single-Cell RNA-Seq Datasets using rna star solo

usegalaxy.org support
,
1

I have two fastq reads (R1 and R2) for single cell rna-seq data.
R1 is 9.5GB and R2 is 19.5 GB. As far as I know R1 is usually the barcode read and R2 is the cDNA read.
I am trying to follow the galaxy pipeline Hands-on: Hands-on: Pre-processing of 10X Single-Cell RNA Datasets / Single Cell

But in this training 4 files are used, two files for each reads. I only have two files, one file per read. I used R1 as barcode read and R2 as cDNA read as the input parameters for rna star solo. But I got an error in generating alignment bam file and all the other files were empty.

Can anyone help me figure out? I am new to this whole thing.

Thanks

2

Resolved in reruns.