# Different versions of trimmomatic gives different output

**URL:** <https://help.galaxyproject.org/t/different-versions-of-trimmomatic-gives-different-output/4728>\
**Category:** Uncategorized\
**Tags:** tool-version, usinggalaxy, quality-control\
**Created:** [October 28, 2020, 9:55am UTC](https://help.galaxyproject.org/t/different-versions-of-trimmomatic-gives-different-output/4728 "2020-10-28T09:55:57Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![bee](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/bee/32/1906_2.png) [@bee](https://help.galaxyproject.org/u/bee)\
**Post date:** [October 28, 2020, 9:55am UTC](https://help.galaxyproject.org/t/different-versions-of-trimmomatic-gives-different-output/4728/1 "2020-10-28T09:55:57Z")

</div>

I’m getting different outputs with the same input using the exact same tool of different versions.  
I have used Trimmomatic version 0.36.5 on [usegalaxy.eu](http://usegalaxy.eu) and Trimmomatic version 0.38.0 on [usegalaxy.org](http://usegalaxy.org). I used the same data as inputs. Only difference was the tool versions. And the output I got after trimming from both versions showed different results.

The number of transcripts decreased drastically after trimming with Trimmomatic version 0.38.0 on [usegalaxy.org](http://usegalaxy.org). But the number of transcripts are the same as before trimming with Trimmomatic version 0.36.5 on [usegalaxy.eu](http://usegalaxy.eu).

Can anyone please help me understand why I’m getting such different outputs with same toll of different versions?
