# Disk quota error occurring with paired-end Trinity assembly -- Sept 22 2021 = Resolved **URL:** \ **Category:** usegalaxy.org support\ **Tags:** assembly, troubleshooting\ **Created:** [September 22, 2021, 6:54pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706 "2021-09-22T18:54:00Z")\ **Posts on this page:** 10\ **Page:** 1
**Author:** ![BirdNerd](https://avatars.discourse-cdn.com/v4/letter/b/9e8a1a/32.png) [@BirdNerd](https://help.galaxyproject.org/u/BirdNerd)\ **Post date:** [September 22, 2021, 6:54pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/1 "2021-09-22T18:54:00Z")
Hi! Our lab uses Trinity for most of our assembly work, but lately, I’ve been unable to get the tool to run correctly. What I am attempting to assemble is a set of paired-end Illumina reads, with a file size of about 360MB for each strand. This created a near-instantaneous error report when the job transitioned from ‘pending’ to ‘running’. The job API ID is bbd44e69cb8906b567f21c7489016a08, and the error code is “[Errno 122] Disk quota exceeded”. My understanding of that error code is that it is a memory issue, but we’ve assembled larger datasets than that in the last without issue. Any clarification you could give would be appreciated. ---
**Author:** ![R\_R\_R](https://avatars.discourse-cdn.com/v4/letter/r/eb9ed0/32.png) [@R\_R\_R](https://help.galaxyproject.org/u/R_R_R)\ **Post date:** [September 22, 2021, 8:25pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/2 "2021-09-22T20:25:53Z")
I had the same issue today. Must be a problem on the server. I sent an error report. ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [September 22, 2021, 8:30pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/3 "2021-09-22T20:30:41Z")
Hi @BirdNerd This error was due to a cluster problem at [UseGalaxy.org](http://UseGalaxy.org) that should be resolved right now `[Errno 122] Disk quota exceeded`. If you are still getting that error from jobs that were started this week it would be unexpected. Please send in a bug report that includes a link to this topic thread in the comments. That said, reviewing your account at this server, the most recent `Trinity` runs have a different error. The complaint from the tool `stdout` is that the fastq inputs were not formatted corrrectly. Common reasons include: data not fully uploaded, gz compression didn’t load correctly, or the data is truncated before uploading to Galaxy. Check the data locally to make sure it is intact, then upload uncompressed fastq and run some QA/QC checks. As you probably know, `Trinity` expects that both ends of a pair are input. I added a few tags to your post that link to prior Q&A that helps with troubleshooting that kind of problem. Thanks! ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [September 22, 2021, 8:32pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/4 "2021-09-22T20:32:40Z")
Hi @R_R_R – Thanks for sending in the bug report. That issue should be fixed but … some data migrations are still going on. We’ll review and post back an update. ---
**Author:** ![BirdNerd](https://avatars.discourse-cdn.com/v4/letter/b/9e8a1a/32.png) [@BirdNerd](https://help.galaxyproject.org/u/BirdNerd)\ **Post date:** [September 22, 2021, 8:34pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/5 "2021-09-22T20:34:23Z")
Okay, thanks for helping with that. I’m less concerned about the second dataset (although I had noticed that it had a different error code), as I just wanted to see if I could replicate the error independent of the Pluvialis data. Thanks for the information, and I’ll try and run it again. ---
**Author:** ![R\_R\_R](https://avatars.discourse-cdn.com/v4/letter/r/eb9ed0/32.png) [@R\_R\_R](https://help.galaxyproject.org/u/R_R_R)\ **Post date:** [September 22, 2021, 8:38pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/6 "2021-09-22T20:38:27Z")
Thanks Jen. I am trying again now with a small dataset (one I know should work) to see if it happens again. If it does, I’ll make sure to send another bug report. ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [September 22, 2021, 8:49pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/7 "2021-09-22T20:49:33Z")
@R_R_R For your run sent in, the job did hit a cluster problem that should now be resolved (was present for a short time today). @BirdNerd Also please try a rerun (with intact fastq). About 20 GB per end (compressed) is the largest I’ve seen assemble at a public Galaxy. Larger work can be moved to a [custom Galaxy](https://help.galaxyproject.org/t/job-failure-due-to-exceeding-resources-at-public-galaxy-servers-solution-modify-the-job-or-consider-a-custom-galaxy-server/6614/2). Thanks to you both for reporting the problem! ---
**Author:** ![R\_R\_R](https://avatars.discourse-cdn.com/v4/letter/r/eb9ed0/32.png) [@R\_R\_R](https://help.galaxyproject.org/u/R_R_R)\ **Post date:** [September 22, 2021, 8:55pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/8 "2021-09-22T20:55:08Z")
Thanks Jen, I actually have run Trinity with a set that were around 160 GB per end and didn’t have a problem (uncompressed, 18 GB compressed). I just double checked it to be sure. I can let you know which data set that was if you want to see it. Most of my Trinities have been over 100 GB uncompressed. ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [September 22, 2021, 9:44pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/9 "2021-09-22T21:44:04Z")
@R_R_R Thanks for clarifying – I updated the original post. Maybe the “large data” info will help someone else 🙂 ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [December 20, 2023, 9:54pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/10 "2023-12-20T21:54:46Z")
Closed on December 20, 2023, 9:54pm UTC