# Disk quota error occurring with paired-end Trinity assembly -- Sept 22 2021 = Resolved

**URL:** <https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706>\
**Category:** usegalaxy.org support\
**Tags:** assembly, troubleshooting\
**Created:** [September 22, 2021, 6:54pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706 "2021-09-22T18:54:00Z")\
**Posts on this page:** 1\
**Showing post:** 7

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [September 22, 2021, 8:49pm UTC](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706/7 "2021-09-22T20:49:33Z")

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@R_R_R

For your run sent in, the job did hit a cluster problem that should now be resolved (was present for a short time today).

@BirdNerd

Also please try a rerun (with intact fastq).

About 20 GB per end (compressed) is the largest I’ve seen assemble at a public Galaxy. Larger work can be moved to a [custom Galaxy](https://help.galaxyproject.org/t/job-failure-due-to-exceeding-resources-at-public-galaxy-servers-solution-modify-the-job-or-consider-a-custom-galaxy-server/6614/2).

Thanks to you both for reporting the problem!

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_[View the full topic](https://help.galaxyproject.org/t/disk-quota-error-occurring-with-paired-end-trinity-assembly-sept-22-2021-resolved/6706)._