# Downloading paired end data

**URL:** <https://help.galaxyproject.org/t/downloading-paired-end-data/6981>\
**Category:** usegalaxy.eu support\
**Tags:** sra\
**Created:** [November 5, 2021, 1:00pm UTC](https://help.galaxyproject.org/t/downloading-paired-end-data/6981 "2021-11-05T13:00:16Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![amir](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/amir/32/539_2.png) [@amir](https://help.galaxyproject.org/u/amir)\
**Post date:** [November 5, 2021, 1:00pm UTC](https://help.galaxyproject.org/t/downloading-paired-end-data/6981/1 "2021-11-05T13:00:16Z")

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Hi  
I have some trouble in downloading paired end datasets.  
After founding datasets i want to download them through **Download and Extract Reads in FASTA/Q** tool. one by one with SRA accession option. But in this case i have just one dataset instead of two for each pair.  
AND option of “list of accession numbers” not working for a while.

Kind regards

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**Author:** ![gbbio](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/gbbio/32/366_2.png) [@gbbio](https://help.galaxyproject.org/u/gbbio)\
**Post date:** [November 5, 2021, 1:42pm UTC](https://help.galaxyproject.org/t/downloading-paired-end-data/6981/2 "2021-11-05T13:42:44Z")

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Is it possible that the files are Interlaced? See:  
[https://galaxyproject.org/support/ncbi-sra-fastq/](https://galaxyproject.org/support/ncbi-sra-fastq/)

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**Author:** ![amir](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/amir/32/539_2.png) [@amir](https://help.galaxyproject.org/u/amir)\
**Post date:** [November 5, 2021, 3:47pm UTC](https://help.galaxyproject.org/t/downloading-paired-end-data/6981/3 "2021-11-05T15:47:18Z")

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Based of what i saw in the link you sent, i dont think that was my problem  
@1/1  
GGCGCGGCCCTTCTCGGCGAGGCGGCGGCGGCGCGGAAGCCGTGGTCCGGGCTCTGGCCGCGGCGCCGGGAGCGACAACGGTTGCGAGCCCGGGGCTCGA  
+  
FFFFFFFFFFFFGFFF=FF@FFEFFFFFFFFFFFFFAFFFFBBFGGFG=FFFEGDFFEFFFBFFFBF@DF/F8FFF@FGFF/FFF9)G7EGBFF\<8GEGE  
@2/1  
CTCTCTCCTCAGCGCTTCTTCTTTCTTGGTTTGATCCTGACTGCTGTCATGGCGTGCCCTCTGGAGAAGGCCCTGGATGTGATGGTGTTCACCTTCCACA  
+  
;FDF:B5\<FC[C8E9FBABCBFAF=EE98EFECBFC7FDC=FFBFCF@EFEBD07D](mailto:C8E9FBABCBFAF=EE98EFECBFC7FDC=FFBFCF@EFEBD07D)FDF:F:?\<@==?D4BDA=D?3\>=A9BF=;4@&AD7\<\>\<;\>:ED  
@3/1  
AGCGGGGGCCCTCAGGGAAGCAACCCAGCTCGCATCTGGAAGGCCAGGCAGTCCCCTCGGGTGGCTTGCTTGTTGATCTCTCTGTGTCGTTCTTTGGTTA  
+  
AEE0BAD=EDEEEEDECB?C54EF:FDEFDDEFDFDEFD0BCEFCEE7EEFDDCEDF\>FEA\>F:CDEF=FEEBFEDDFECFCE.EFEEFE0:FECEDF?F  
@4/1

this is example of the reads and it is different from what is mentioned about Interlaced reads

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<div class="post-metadata">

**Author:** ![amir](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/amir/32/539_2.png) [@amir](https://help.galaxyproject.org/u/amir)\
**Post date:** [November 5, 2021, 3:50pm UTC](https://help.galaxyproject.org/t/downloading-paired-end-data/6981/4 "2021-11-05T15:50:17Z")

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My problem is : I found a dataset which is not available in ENA but its available in SRA file. and because it is paired end, I don’t know how i can download it.