I am running into this error when I try to do differential seq analysis in galaxy:
Warning message:
In estimateDisp.default(y = y$counts, design = design, group = group, :
No residual df: setting dispersion to NA
Error in eval(ej, envir = levelsenv) :
object ‘ControlvsTreated’ not found
Calls: makeContrasts → eval → eval
@Deepika_Chandrasekar
Have you searched the forum?
https://www.biostars.org/p/212200/
Does it answer the question?
Kind regards,
Igor
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