# Error in de novo assembly of RNAseq using Trinity

**URL:** <https://help.galaxyproject.org/t/error-in-de-novo-assembly-of-rnaseq-using-trinity/6893>\
**Category:** usegalaxy.org support\
**Tags:** assembly, troubleshooting\
**Created:** [October 20, 2021, 2:09pm UTC](https://help.galaxyproject.org/t/error-in-de-novo-assembly-of-rnaseq-using-trinity/6893 "2021-10-20T14:09:20Z")\
**Posts on this page:** 1\
**Showing post:** 6

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [October 21, 2021, 2:56pm UTC](https://help.galaxyproject.org/t/error-in-de-novo-assembly-of-rnaseq-using-trinity/6893/6 "2021-10-21T14:56:23Z")

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Hi @andre.sa

Try uncompressing the fastq reads before running `Trinity`.

Pencil icon \> Convert \> uncompress. Do this on each output from the `Fastq Splitter` tool or on the original dataset 8, then split.

This post has more details about general usage help for Trinity including tutorial links: [too much time for start a job? Troubleshooting Trinity inputs + QA/QC steps for RNA-seq assembly - #4 by jennaj](https://help.galaxyproject.org/t/too-much-time-for-start-a-job-troubleshooting-trinity-inputs-qa-qc-steps-for-rna-seq-assembly/4951/4)

Thanks!

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