# Error in Format Data for LEfSe

**URL:** <https://help.galaxyproject.org/t/error-in-format-data-for-lefse/7746>\
**Category:** Uncategorized\
**Tags:** galaxy-biobakery\
**Created:** [March 25, 2022, 4:45am UTC](https://help.galaxyproject.org/t/error-in-format-data-for-lefse/7746 "2022-03-25T04:45:59Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![ivanllampy](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/ivanllampy/32/3230_2.png) [@ivanllampy](https://help.galaxyproject.org/u/ivanllampy)\
**Post date:** [March 25, 2022, 4:45am UTC](https://help.galaxyproject.org/t/error-in-format-data-for-lefse/7746/1 "2022-03-25T04:45:59Z")

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Hi all,  
I am a new user in Galaxy, I am trying to import my 16S dataset for LEfSe.  
Here is the layout of my dataset:

 ![database](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/b/bf868c4730ca2620814741637dc193bcb8123e8e.png)  
An error then occurs at the “Format Data for LEfSe” stage, as below:  
 ![error](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/6/6cf18a0fa8449be341c7c8af4123e2f1595e870f.png)  
I have tried using the Galaxy sever and the command line version, both got the same error.

Would it be a problem of my data format?  
I noticed a similar problem in ([Problems with format data for LEfSe](https://help.galaxyproject.org/t/problems-with-format-data-for-lefse/5847)), but have no idea how to fix it.

Here also attach the Galaxy layout I selected for reference, not sure if relevant or not:

 ![Galaxy layout](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/d/dd41e3f50f0fcca5a61c6e1da525c486177f5044.png)

In addition, I would also like to ask would negative values (coming from clr-transformation of dataset) be acceptable in LEfSe? The dataset used above has no negative values.

Many thanks for your help, highly appreciate!

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**Author:** ![allansantos](https://avatars.discourse-cdn.com/v4/letter/a/4bbf92/32.png) [@allansantos](https://help.galaxyproject.org/u/allansantos)\
**Post date:** [May 3, 2024, 8:52pm UTC](https://help.galaxyproject.org/t/error-in-format-data-for-lefse/7746/2 "2024-05-03T20:52:00Z")

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Hi  
Did you get solve this?

thank you

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [May 3, 2024, 11:11pm UTC](https://help.galaxyproject.org/t/error-in-format-data-for-lefse/7746/3 "2024-05-03T23:11:37Z")

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Please see

> [@Troubleshooting resources for errors or unexpected results](https://help.galaxyproject.org/t/troubleshooting-resources-for-errors-or-unexpected-results/42/1):
>
> #### Alternative support forums
> 
> When working at certain Galaxy sites, questions are best asked at the places the scientists who run the server host discussions. They can also let you know if there happen to be any current technical problems that may be impacting your analysis. These are the ones we know about…
> 
> 🥯 **[Galaxy.bioBakery.org](http://Galaxy.bioBakery.org)** → [https://forum.biobakery.org](https://forum.biobakery.org)  
> This server was previously hosted at [http://huttenhower.sph.harvard.edu/galaxy/](http://huttenhower.sph.harvard.edu/galaxy/), and prior Q&A here is tagged #galaxy-huttenhower

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<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [January 16, 2025, 6:06pm UTC](https://help.galaxyproject.org/t/error-in-format-data-for-lefse/7746/4 "2025-01-16T18:06:56Z")

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