# Error of Deseq2

**URL:** https://help.galaxyproject.org/t/error-of-deseq2/10977
**Category:** usegalaxy.eu support
**Tags:** mapping, transcriptomics, deseq2, stringtie
**Created:** [October 15, 2023, 11:52am UTC](https://help.galaxyproject.org/t/error-of-deseq2/10977 "2023-10-15T11:52:45Z")
**Posts on this page:** 1
**Showing post:** 2

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### Author: ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)
#### Post date: [October 16, 2023, 7:07pm UTC](https://help.galaxyproject.org/t/error-of-deseq2/10977/2 "2023-10-16T19:07:48Z")

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Hello @hashimoto.takuma

This tool and most other Bioconductor tools require replicates.

FAQ with related tips: [Extended Help for Differential Expression Analysis Tools](https://training.galaxyproject.org/training-material/faqs/galaxy/analysis_differential_expression_help.html)

Give that a review and maybe reorganize your sample data?

If you run into more problems, you can post back the job details and we can try to help more. The part with the inputs and parameters is the most important (“i” icon). Try to leave everything intact with a full copy/paste or screenshot.

> [@Getting Data into Galaxy](https://help.galaxyproject.org/t/getting-data-into-galaxy/10868/1):
>
> #### 🧑‍🔧 [What information should I include when reporting a problem?](https://training.galaxyproject.org/training-material/faqs/galaxy/analysis_troubleshooting_reporting.html)
> 
> Any persistent problems can be reported in a new question for community help. Be sure to provide enough context so others can review the situation _exactly_ and quickly offer advice.
> 
> Consider [Sharing your History](https://training.galaxyproject.org/training-material/faqs/galaxy/histories_sharing.html) or posting content from the Job Information ℹ view as described in [Troubleshooting errors](https://training.galaxyproject.org/training-material/faqs/galaxy/analysis_troubleshooting.html).

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