# Error when running "Trimmomatic flexible read trimming tool for Illumina NGS data" on RNA seq data

**URL:** <https://help.galaxyproject.org/t/error-when-running-trimmomatic-flexible-read-trimming-tool-for-illumina-ngs-data-on-rna-seq-data/8391>\
**Category:** usegalaxy.eu support\
**Tags:** troubleshooting\
**Created:** [July 31, 2022, 12:48pm UTC](https://help.galaxyproject.org/t/error-when-running-trimmomatic-flexible-read-trimming-tool-for-illumina-ngs-data-on-rna-seq-data/8391 "2022-07-31T12:48:24Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![AU-postdoc](https://avatars.discourse-cdn.com/v4/letter/a/bb73d2/32.png) [@AU-postdoc](https://help.galaxyproject.org/u/AU-postdoc)\
**Post date:** [July 31, 2022, 12:48pm UTC](https://help.galaxyproject.org/t/error-when-running-trimmomatic-flexible-read-trimming-tool-for-illumina-ngs-data-on-rna-seq-data/8391/1 "2022-07-31T12:48:24Z")

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Hellow everyone  
I work with my colleagues on RNA-seq human data where we are trying to analyze these data on [usegalaxy.eu](http://usegalaxy.eu). we have +50 raw single-end sequencing data files. We have started our analysis by running FastQC on all data files with no problem. Now we are trying to run “Trimmomatic flexible read trimming tool for Illumina NGS data” on the data files to cut the first 5 bases but we are getting the error in attached image for 8 of these data files. We have tried to run these 8 data files on [usegalaxy.org](http://usegalaxy.org) to see if the files will run correctly but we got the same error. Hope you can help us to troubleshoot this problem.

 ![IMG_20220728_093632](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/a/ac124b0e32b62da31596cba7601c2e29ee7df4c2.jpeg)

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**Author:** ![AU-postdoc](https://avatars.discourse-cdn.com/v4/letter/a/bb73d2/32.png) [@AU-postdoc](https://help.galaxyproject.org/u/AU-postdoc)\
**Post date:** [August 10, 2022, 2:37pm UTC](https://help.galaxyproject.org/t/error-when-running-trimmomatic-flexible-read-trimming-tool-for-illumina-ngs-data-on-rna-seq-data/8391/2 "2022-08-10T14:37:27Z")

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Problem solved

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [August 22, 2022, 5:50pm UTC](https://help.galaxyproject.org/t/error-when-running-trimmomatic-flexible-read-trimming-tool-for-illumina-ngs-data-on-rna-seq-data/8391/3 "2022-08-22T17:50:29Z")

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