# Error while performing LEfSe LDA Effect size

**URL:** <https://help.galaxyproject.org/t/error-while-performing-lefse-lda-effect-size/14196>\
**Category:** usegalaxy.org support\
**Tags:** galaxy-biobakery\
**Created:** [December 17, 2024, 11:12am UTC](https://help.galaxyproject.org/t/error-while-performing-lefse-lda-effect-size/14196 "2024-12-17T11:12:17Z")\
**Posts on this page:** 1\
**Showing post:** 2

<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [December 17, 2024, 9:52pm UTC](https://help.galaxyproject.org/t/error-while-performing-lefse-lda-effect-size/14196/2 "2024-12-17T21:52:58Z")

</div>

> [@Zbourhane](#):
>
> `AttributeError: 'NoneType' object has no attribute 'rownames'`

Welcome, @Zbourhane

This is your question at the other forum, yes? Keep following up where you are right now at → [LEfSe issues and errors - #38 by Zee - LEfSe - The bioBakery help forum](https://forum.biobakery.org/t/lefse-issues-and-errors/5397/38)

The problems with this tool seem to be server side, and those scientists are the only people who can fix it. They want people to use the updated pipeline for both scientific and technical reasons – read about that in their other topics.

For everyone who has a problem at the **Biobakery Galaxy** server, we have this message to share. We would like to help but _can’t_ in any practical way due to the unique nature of that server’s “wrapped tools” and how the server is hosted.

> [@Troubleshooting resources for errors or unexpected results](https://help.galaxyproject.org/t/troubleshooting-resources-for-errors-or-unexpected-results/42/1):
>
> #### Alternative support forums
> 
> When working at certain Galaxy sites, questions are best asked at the places the scientists who run the server host discussions. They can also let you know if there happen to be any current technical problems that may be impacting your analysis. These are the ones we know about…
> 
> 🥯 **[Galaxy.bioBakery.org](http://Galaxy.bioBakery.org)** → [https://forum.biobakery.org](https://forum.biobakery.org)  
> This server was previously hosted at [Galaxy](http://huttenhower.sph.harvard.edu/galaxy/), and prior Q&A here is tagged #galaxy-huttenhower

So, since those wrapped tools are unique and different from the other public Galaxy servers, you could also explore the alternative Lefse tools as part of the Mothur pipeline at one of the UseGalaxy servers. It works a bit differently but maybe interesting anyway – and the original tool publication would apply. Search the tool panel with the tool name to find it. Search the GTN tutorials and workflows for how to prepare the data for that kind of pipeline (keyword “mothur”). Lefse isn’t covered specifically but the tutorials will help to get your data organized and ready.

Hope this helps!

Prior Q&A here → [How to fix errors with Lefse - #4 by jennaj](https://help.galaxyproject.org/t/how-to-fix-errors-with-lefse/10183/4)

---

_[View the full topic](https://help.galaxyproject.org/t/error-while-performing-lefse-lda-effect-size/14196)._