# Errors with Humann and Interproscan

**URL:** <https://help.galaxyproject.org/t/errors-with-humann-and-interproscan/11472>\
**Category:** usegalaxy.org.au support\
**Tags:** metagenomics, interproscan, humann\
**Created:** [January 13, 2024, 12:28pm UTC](https://help.galaxyproject.org/t/errors-with-humann-and-interproscan/11472 "2024-01-13T12:28:02Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![vebaev](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/vebaev/32/843_2.png) [@vebaev](https://help.galaxyproject.org/u/vebaev)\
**Post date:** [January 13, 2024, 12:28pm UTC](https://help.galaxyproject.org/t/errors-with-humann-and-interproscan/11472/1 "2024-01-13T12:28:02Z")

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I got errors for a jobs with Humann and interproscan, I reported them and also I’m pasting my history here:  
[https://usegalaxy.org.au/u/baev/h/metabac](https://usegalaxy.org.au/u/baev/h/metabac)

Can someone advice it why the jobs have been erroring 🙂

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [January 16, 2024, 7:17pm UTC](https://help.galaxyproject.org/t/errors-with-humann-and-interproscan/11472/2 "2024-01-16T19:17:51Z")

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Hi @vebaev

**HUMAnN**

The job is erroring with this message in the Standard Output log (aka `stdout`).

> WARNING: Can not call software version for bowtie2

If there is a problem with the fastq reads, that can lead to unusual problems with _any_ tool. I would try running some QA on the reads. Address items like: Is the datatype correct? Are the pairs intact? Tutorials are linked at the bottom of the tool form, and those have example data. Search that same site with “quality” for QA tutorials.

**InterProScan**

Logs are also in the `stdout` for this one.

> You have submitted a protein sequence which contains an asterix (_). This may be from an ORF prediction program. '_’ is not a valid IUPAC amino acid character and amino acid sequences which go through our pipeline should not contain it. Please strip out all asterix characters from your sequence and resubmit your search.

For that, you’ll need to adjust the input data as the author instructed for it to work anywhere.