# Extract CuffDiff tool

**URL:** <https://help.galaxyproject.org/t/extract-cuffdiff-tool/11671>\
**Category:** usegalaxy.org.au support\
**Tags:** tool-deprecated\
**Created:** [February 7, 2024, 5:52am UTC](https://help.galaxyproject.org/t/extract-cuffdiff-tool/11671 "2024-02-07T05:52:49Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Ahmad\_Suparmin](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/ahmad_suparmin/32/5164_2.png) [@Ahmad\_Suparmin](https://help.galaxyproject.org/u/Ahmad_Suparmin)\
**Post date:** [February 7, 2024, 5:52am UTC](https://help.galaxyproject.org/t/extract-cuffdiff-tool/11671/1 "2024-02-07T05:52:49Z")

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I can not find Extract CuffDiff tool in galaxy australia, so any help to find or alternative way to extract RNA-Seq SQLite result data?

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [February 8, 2024, 6:30pm UTC](https://help.galaxyproject.org/t/extract-cuffdiff-tool/11671/2 "2024-02-08T18:30:16Z")

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Hello @Ahmad_Suparmin

The **CuffDiff** tool and most others from the original Tuxedo pipeline have been deprecated. That means they won’t work on the current releases of Galaxy.

Alternative differential expression tools are covered in the GTN tutorials →

- RNA-seq [Transcriptomics / Tutorial List](https://training.galaxyproject.org/training-material/topics/transcriptomics/)
- Single-cell [Single Cell / Tutorial List](https://training.galaxyproject.org/training-material/topics/single-cell/)

These do not create a “database” of the data but instead work with flat files in tabular formats.