Hi, my name is Julia. Im new to Galaxy NGS and want to edit my Fastq files: I would like to crop bases from the beginning of the read and from the end of the read. As far as Ive seen, there are only fastq tools, which allow me to crop fastq files depending on their quality scores.
I want to crop fastq files independently from the quality scores.
Thanks for your help in advance!
Julia
You can use the " Trim sequences" tool to trim from the beginning and end of the read. So e.g. if you have 100 bp reads, and set “First based to keep” to 10 and “Last base to keep” to 85" you will end up with 75 bp reads - from the 10th base to the 85th base.
I hope this helps.
When I want to add the fastq File into the trim sequence application it says: (unavailable) and then the name of the file. And the analysis doesn’t work, what could be the reason for that?
That would typically mean that the FASTQ has not uploaded correctly?