# Fatal error: Exit code 1 () MultiQC and STAR erros

**URL:** <https://help.galaxyproject.org/t/fatal-error-exit-code-1-multiqc-and-star-erros/5452>\
**Category:** usegalaxy.org support\
**Tags:** multiqc, transcriptomics, reference-genome, rna\_star\
**Created:** [February 25, 2021, 2:33pm UTC](https://help.galaxyproject.org/t/fatal-error-exit-code-1-multiqc-and-star-erros/5452 "2021-02-25T14:33:47Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Mirianfp](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/mirianfp/32/2184_2.png) [@Mirianfp](https://help.galaxyproject.org/u/Mirianfp)\
**Post date:** [February 25, 2021, 2:33pm UTC](https://help.galaxyproject.org/t/fatal-error-exit-code-1-multiqc-and-star-erros/5452/1 "2021-02-25T14:33:47Z")

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Hi, I am following the Reference-based RNA-Seq data analysis tutorial to analyze a dual RNA seq with fungal transcriptions. I downloaded the ref genome fasta files from ensemble with respective annotations. While running STAR I am getting errors for some of my samples :

!!! WARNING: --genomeSAindexNbases 14 is too large for the genome size=40980648, which may cause seg-fault at the mapping step. Re-run genome generation with recommended --genomeSAindexNbases 11

EXITING because of FATAL ERROR: number of bytes expected from the BAM bin does not agree with the actual size on disk: Expected bin size=175717184 ; size on disk=168213990 ; bin number=47

Feb 25 05:56:38 … FATAL ERROR, exiting

For some of the samples the already ran STAR and worked, I am using multiQC to aggregate STAR logs, getting more errors:  
Fatal error: Exit code 1 ()  
ln: failed to create symbolic link ‘multiqc\_WDir/star\_0/log\_0/RNA STAR on data 770\_ data 771\_ and others\_ log\_Log.final.out’: File exists

Anybody can help please?

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**Author:** ![gallardoalba](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/gallardoalba/32/1903_2.png) [@gallardoalba](https://help.galaxyproject.org/u/gallardoalba)\
**Post date:** [March 3, 2021, 2:35pm UTC](https://help.galaxyproject.org/t/fatal-error-exit-code-1-multiqc-and-star-erros/5452/2 "2021-03-03T14:35:49Z")

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Hi @Mirianfp,  
could you repeat the alignment by using a higher value in the **Number of genome bins for coordinate-sorting** parameter?

Regards