FeaturCount Error

1

Hi, I’m a beginner using Galaxy. I analyzed paired-end data and obtained mapping results. However, when I ran featureCounts, I encountered the following error:

"ERROR: failed to find the gene identifier attribute in the 9th column of the provided GTF file. The specified gene identifier attribute is ‘gene_id’. An example of attributes included in your GTF annotation is ‘exon ENSMUST00000193812.2;’."

How can I resolve this issue?

2

Hi @Atefeh_Bahmei,

similar issues were discussed on this forum multiple times, just search it. Most likely, you can resolve the issue with settings matching the annotation file.

Kind regards,

Igor

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