Hello,
I was able to fix the featurecounts error and now I have an error in deseq2. Below is the error. It would be great if you could help me with this.
Fatal error: An undefined error occurred, please check your input carefully and contact your administrator.
Tool generated the following standard error:
estimating size factors
estimating dispersions
Error in checkForExperimentalReplicates(object, modelMatrix) :
The design matrix has the same number of samples and coefficients to fit,
so estimation of dispersion is not possible. Treating samples
as replicates was deprecated in v1.20 and no longer supported since v1.22.
Calls: DESeq ... estimateDispersions -> .local -> checkForExperimentalReplicates