I’m trying to install a custom blast db into a locally hosted instance of Galaxy. I have edited the blastdb.loc file and it is properly detected by Galaxy, as seen in the admin datatable:
The file path points to a folder with the following files:
mfas.nhr mfas.nin mfas.nog mfas.nsd mfas.nsi mfas.nsq.
If I go to that folder and run blast at the command line using the “mfas” keyword, the database is fine.
However, when I run the NCBI BLAST + blastn Galaxy tool, I get this error:
BLAST Database error: No alias or index file found for nucleotide database [/apps/galaxy/galaxy_staging1/tool-data/app_db/mfas/] in search path [/apps/galaxy/galaxy_staging1/database/jobs_directory/000/9/working::]
What am I doing wrong? I am currently using the full filepath but I don’t think it’s pointing correctly. I’ve read the tutorials but it’s unclear if I’m supposed to provide the full filepath or a relative filepath.
Any help would be greatly appreciated! I’m new to Galaxy admin but will need to install quite a few custom DBs for my group so I really need this working.