# Generate spectral library using diapysef without iRT file

**URL:** <https://help.galaxyproject.org/t/generate-spectral-library-using-diapysef-without-irt-file/6500>\
**Category:** usegalaxy.eu support\
**Tags:** gtn-tutorial\
**Created:** [August 11, 2021, 2:45pm UTC](https://help.galaxyproject.org/t/generate-spectral-library-using-diapysef-without-irt-file/6500 "2021-08-11T14:45:46Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![Matthijs\_dg](https://avatars.discourse-cdn.com/v4/letter/m/c6cbf5/32.png) [@Matthijs\_dg](https://help.galaxyproject.org/u/Matthijs_dg)\
**Post date:** [August 11, 2021, 2:45pm UTC](https://help.galaxyproject.org/t/generate-spectral-library-using-diapysef-without-irt-file/6500/1 "2021-08-11T14:45:46Z")

</div>

Hi,

I’m trying to generate a spectral library with our DDA data, that have been quantified with Maxquant, using the diapysef tool as is described in this tutorial ([Library Generation for DIA Analysis](https://training.galaxyproject.org/training-material//topics/proteomics/tutorials/DIA_lib_OSW/tutorial.html#diapysef-library-generation)).  
As we don’t have an iRT file for these runs, is it possible to run this tool without the iRT-file?

Thanks, Matt