# GFF to FASTA - Problems Converting Files

**URL:** <https://help.galaxyproject.org/t/gff-to-fasta-problems-converting-files/4217>\
**Category:** Uncategorized\
**Created:** [July 31, 2020, 7:44pm UTC](https://help.galaxyproject.org/t/gff-to-fasta-problems-converting-files/4217 "2020-07-31T19:44:27Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Ryan\_Morris](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/ryan_morris/32/493_2.png) [@Ryan\_Morris](https://help.galaxyproject.org/u/Ryan_Morris)\
**Post date:** [July 31, 2020, 7:44pm UTC](https://help.galaxyproject.org/t/gff-to-fasta-problems-converting-files/4217/1 "2020-07-31T19:44:27Z")

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Hi All,

I have recently the following file from ensembl to use as a reference genome for quantifying RNA-seq transcripts via Kallisto; Homo\_sapiens.GRCh38.83.chr.gtf.gz

Once I have uploaded this file to the galaxy server the file as read as a GFF file. For kallisto quantification I need to have this file as a FASTA file. How would I be able to convert from the GFF format to FASTA

Ryan