# Heatmaps of DEGs presented as z-scores/RNA-seq

**URL:** <https://help.galaxyproject.org/t/heatmaps-of-degs-presented-as-z-scores-rna-seq/13173>\
**Category:** usegalaxy.org support\
**Tags:** rna-seq\
**Created:** [August 8, 2024, 2:05pm UTC](https://help.galaxyproject.org/t/heatmaps-of-degs-presented-as-z-scores-rna-seq/13173 "2024-08-08T14:05:52Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![Manolis1](https://avatars.discourse-cdn.com/v4/letter/m/977dab/32.png) [@Manolis1](https://help.galaxyproject.org/u/Manolis1)\
**Post date:** [August 8, 2024, 2:05pm UTC](https://help.galaxyproject.org/t/heatmaps-of-degs-presented-as-z-scores-rna-seq/13173/1 "2024-08-08T14:05:52Z")

</div>

Hello,

I have used DESeq2 to identify DEGs. What I would like to do next, is to generate a heatmap (via the heatmap2 tool), where I should show those DEGs (cut off: p\<00,5 & -1\<Log2(FC)\>1), presented as z-scores. From a GALAXY tutorial that I have found ([Hands-on: Reference-based RNA-Seq data analysis / Reference-based RNA-Seq data analysis / Transcriptomics](https://training.galaxyproject.org/training-material/topics/transcriptomics/tutorials/ref-based/tutorial.html)), I understood that I cannot use directly the `rLog2 Normalized counts` file that the DESeq2 produces, but instead I should follow a process (not so clear to me, as it is presented in this tutorial), in order to create another file that I can then use it as input to the Heatmap2 tool in order to produce the heatmap that I`m wishing, with the z-scores of the DEGs.  
Could you please help me to understand what type of file should I use, as input, in the Heatmap2 tool in order to produce a heatmap with DEG, presenting as z-scores? Will it be ok if I use the `rLog-Normalized counts` from DEseq directly? If not, then what kind of file should I use, and how can I produce it (process, tools, inputs).  
I would highly appreciate any help,

Cheers,

Manolis