# HELP with Unicycler. I keep getting the error "Remote job server indicated a problem running or monitoring this job"

**URL:** <https://help.galaxyproject.org/t/help-with-unicycler-i-keep-getting-the-error-remote-job-server-indicated-a-problem-running-or-monitoring-this-job/3959>\
**Category:** usegalaxy.org support\
**Tags:** assembly, troubleshooting\
**Created:** [June 22, 2020, 4:40pm UTC](https://help.galaxyproject.org/t/help-with-unicycler-i-keep-getting-the-error-remote-job-server-indicated-a-problem-running-or-monitoring-this-job/3959 "2020-06-22T16:40:10Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![pax](https://avatars.discourse-cdn.com/v4/letter/p/f0a364/32.png) [@pax](https://help.galaxyproject.org/u/pax)\
**Post date:** [June 22, 2020, 4:40pm UTC](https://help.galaxyproject.org/t/help-with-unicycler-i-keep-getting-the-error-remote-job-server-indicated-a-problem-running-or-monitoring-this-job/3959/1 "2020-06-22T16:40:10Z")

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Hello,

Over the weekend, I tried to assemble a phage genome using Unicycler from a 150cycles Miseq data (not paired end) and an oxford nanopore run.

I keep getting the message “Remote job server indicated a problem running or monitoring this job.”. My guess is that my input files are wrong. For the illumina data, I am entering the fastq filed returned by the Miseq which galaxy recognizes as fastqsanger. The nanopore data was a bunch of different fastq files that are not recognized as fastqsanger by galaxy. I combined all the fastq files from the nanopore into one fastq file using the “Text Manipulation -\> Concatenate datasets tail-to-head”. So for unicycler, I entered the one fastq from my Miseq run and the combined fastq file from the nanopore and every single time I get the error message "“Remote job server indicated a problem running or monitoring this job”.

Can someone please help me with this ? I am new with all these tools.

Thanks

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [July 10, 2020, 10:58pm UTC](https://help.galaxyproject.org/t/help-with-unicycler-i-keep-getting-the-error-remote-job-server-indicated-a-problem-running-or-monitoring-this-job/3959/2 "2020-07-10T22:58:11Z")

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Q&A follow-up in this post: [Keep getting an error when trying to use CANU assembler with Oxford Nanopore reads](https://help.galaxyproject.org/t/keep-getting-an-error-when-trying-to-use-canu-assembler-with-oxford-nanopore-reads/4027)

Closing this thread

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [July 10, 2020, 10:58pm UTC](https://help.galaxyproject.org/t/help-with-unicycler-i-keep-getting-the-error-remote-job-server-indicated-a-problem-running-or-monitoring-this-job/3959/3 "2020-07-10T22:58:15Z")

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