# HISAT2 Tool Failing: Guidance for known issue at UseGalaxy.org Feb 9 2026. README

**URL:** <https://help.galaxyproject.org/t/hisat2-tool-failing-guidance-for-known-issue-at-usegalaxy-org-feb-9-2026-readme/17436>\
**Category:** usegalaxy.org support\
**Tags:** server-open-issue, hisat2\
**Created:** [February 10, 2026, 6:30am UTC](https://help.galaxyproject.org/t/hisat2-tool-failing-guidance-for-known-issue-at-usegalaxy-org-feb-9-2026-readme/17436 "2026-02-10T06:30:56Z")\
**Posts on this page:** 1\
**Showing post:** 2

<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [February 10, 2026, 7:13pm UTC](https://help.galaxyproject.org/t/hisat2-tool-failing-guidance-for-known-issue-at-usegalaxy-org-feb-9-2026-readme/17436/2 "2026-02-10T19:13:22Z")

</div>

Welcome @axao

Thank you for reporting the error! Hopefully we can help!

### Issue

This error with HISAT2 will present for some jobs and not others, and only at [UseGalaxy.org](http://UseGalaxy.org).

We are working to correct the **jetstream2** cluster configuration server side issue. The job container is incorrect. **HISAT2** and **Mutect2** are both impacted.

### What to do

Reruns are working for me know, but the issue is not confirmed to be corrected yet.

You can also try a rerun if you would like, then let us know the status back here.

**Remember:** if your original job was part of a collection, or if it was executed with a workflow, there are additional toggles on the [rerun](https://galaxyproject.github.io/training-material/faqs/galaxy/datasets_icons.html) tool form to replace results or to continue with the workflow, or both!

- [Rerunning only failed jobs in a workflow: Replace and Resume functions - #2 by jennaj](https://help.galaxyproject.org/t/rerunning-only-failed-jobs-in-a-workflow-replace-and-resume-functions/16387/2)

We’ll post more feedback here once fully confirmed to be resolved! 🙂

---

_[View the full topic](https://help.galaxyproject.org/t/hisat2-tool-failing-guidance-for-known-issue-at-usegalaxy-org-feb-9-2026-readme/17436)._
