# How can i use SILVA\_138.2 latets as a reference to align.seqs tool in GALAXY Server?

**URL:** <https://help.galaxyproject.org/t/how-can-i-use-silva-138-2-latets-as-a-reference-to-align-seqs-tool-in-galaxy-server/13599>\
**Category:** usegalaxy.eu support\
**Tags:** gtn-tutorial, metagenomics, reference-annotation, tool-help\
**Created:** [October 4, 2024, 12:00pm UTC](https://help.galaxyproject.org/t/how-can-i-use-silva-138-2-latets-as-a-reference-to-align-seqs-tool-in-galaxy-server/13599 "2024-10-04T12:00:30Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Ahmer](https://avatars.discourse-cdn.com/v4/letter/a/a3d4f5/32.png) [@Ahmer](https://help.galaxyproject.org/u/Ahmer)\
**Post date:** [October 4, 2024, 12:00pm UTC](https://help.galaxyproject.org/t/how-can-i-use-silva-138-2-latets-as-a-reference-to-align-seqs-tool-in-galaxy-server/13599/1 "2024-10-04T12:00:30Z")

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Hello, I am doing 16s rRNA (V5-V7 region) metagenomics analysis. I downloaded SILVA\_138.2\_SSURef\_NR99\_tax\_silva.fasta.gz from ([https://www.arb-silva.de/fileadmin/silva\_databases/release\_138\_2/Exports/SILVA\_138.2\_SSURef\_NR99\_tax\_silva.fasta.gz](https://www.arb-silva.de/fileadmin/silva_databases/release_138_2/Exports/SILVA_138.2_SSURef_NR99_tax_silva.fasta.gz)). Uploaded its FASTA file as a reference in align.seqs but getting an error. Can someone help with that?

Thanks and regards

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [October 4, 2024, 6:03pm UTC](https://help.galaxyproject.org/t/how-can-i-use-silva-138-2-latets-as-a-reference-to-align-seqs-tool-in-galaxy-server/13599/2 "2024-10-04T18:03:33Z")

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Consolidated into a single post → [How to add SILVA\_138.2 latets as a reference to align.seqs tool in GALAXY Server? - #2 by jennaj](https://help.galaxyproject.org/t/how-to-add-silva-138-2-latets-as-a-reference-to-align-seqs-tool-in-galaxy-server/13597/2)

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [October 4, 2024, 6:03pm UTC](https://help.galaxyproject.org/t/how-can-i-use-silva-138-2-latets-as-a-reference-to-align-seqs-tool-in-galaxy-server/13599/3 "2024-10-04T18:03:37Z")

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