How remove non Standard nucleotide ?

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#1

I have a hairpin dataset and i cant convert it with RNA to DNA convertor because this dataset has some things like Y or R in their sequence, So i cant remove them one by one, is there any tool which can do it all together.
For example clear all sequence except the ACTGactgNn ?

#2

You could use the ‘Text transformation with sed’ tool with this SED Program

/^[^>]/s/[^ACTGNactgn]+//g

This will remove all non-ACTGnactgn characters from all non-header lines in FASTA.

1 Like
#3

Thanks :heart_eyes: