# How to Add gene information (gene name, strand etc.,) to the DESEQ2 output file generated by Galaxy

**URL:** <https://help.galaxyproject.org/t/how-to-add-gene-information-gene-name-strand-etc-to-the-deseq2-output-file-generated-by-galaxy/209>\
**Category:** Uncategorized\
**Created:** [December 16, 2018, 11:26am UTC](https://help.galaxyproject.org/t/how-to-add-gene-information-gene-name-strand-etc-to-the-deseq2-output-file-generated-by-galaxy/209 "2018-12-16T11:26:14Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![biologist](https://avatars.discourse-cdn.com/v4/letter/b/e79b87/32.png) [@biologist](https://help.galaxyproject.org/u/biologist)\
**Post date:** [December 16, 2018, 11:26am UTC](https://help.galaxyproject.org/t/how-to-add-gene-information-gene-name-strand-etc-to-the-deseq2-output-file-generated-by-galaxy/209/1 "2018-12-16T11:26:14Z")

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Dear Admin,

I would like to add gene information (gene name, strand, transcript IDs, uniprot IDs etc) to the DESEQ2 output file generated by Galaxy (EU).  
Please check the attached slide picture.

Is there a way to create it using tools in galaxy or using R ?

[![GALAXY%20QUESTION](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/1X/7fbc53258fcffea866b67823599a355abd5096ca.png)  
GALAXY QUESTION.png960x720 344 KB  
 ](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/1X/7fbc53258fcffea866b67823599a355abd5096ca.png)

.

Thanks in advance.  
James PC

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**Author:** ![pavanvidem](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/pavanvidem/32/1687_2.png) [@pavanvidem](https://help.galaxyproject.org/u/pavanvidem)\
**Post date:** [December 17, 2018, 10:41am UTC](https://help.galaxyproject.org/t/how-to-add-gene-information-gene-name-strand-etc-to-the-deseq2-output-file-generated-by-galaxy/209/2 "2018-12-17T10:41:11Z")

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Dear James,  
You can use [https://usegalaxy.eu/root?tool\_id=toolshed.g2.bx.psu.edu/repos/iuc/deg\_annotate/deg\_annotate/1.0](https://usegalaxy.eu/root?tool_id=toolshed.g2.bx.psu.edu/repos/iuc/deg_annotate/deg_annotate/1.0) for this purpose. At the moment, it can add gene position, gene symbol and biotype from a GTF file but not uniprot, pathways and GO terms.

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**Author:** ![biologist](https://avatars.discourse-cdn.com/v4/letter/b/e79b87/32.png) [@biologist](https://help.galaxyproject.org/u/biologist)\
**Post date:** [January 3, 2019, 3:47pm UTC](https://help.galaxyproject.org/t/how-to-add-gene-information-gene-name-strand-etc-to-the-deseq2-output-file-generated-by-galaxy/209/3 "2019-01-03T15:47:02Z")

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Hi Pavan, Thank you very much for the input. It worked.

 ![image](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/1X/bfc97839aedc81081d52bd9fc0d7abb59825b8da.png)

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**Author:** ![biologist](https://avatars.discourse-cdn.com/v4/letter/b/e79b87/32.png) [@biologist](https://help.galaxyproject.org/u/biologist)\
**Post date:** [January 3, 2019, 3:48pm UTC](https://help.galaxyproject.org/t/how-to-add-gene-information-gene-name-strand-etc-to-the-deseq2-output-file-generated-by-galaxy/209/4 "2019-01-03T15:48:31Z")

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While extracting Workflow, recently i get this error multiple times.  
Could you tell what might be the reason ?

![image](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/1X/1c5eefc7b83bbc8c4b7c8a1d4b3c8a818ffe13f7.png)

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**Author:** ![biologist](https://avatars.discourse-cdn.com/v4/letter/b/e79b87/32.png) [@biologist](https://help.galaxyproject.org/u/biologist)\
**Post date:** [January 3, 2019, 4:27pm UTC](https://help.galaxyproject.org/t/how-to-add-gene-information-gene-name-strand-etc-to-the-deseq2-output-file-generated-by-galaxy/209/5 "2019-01-03T16:27:31Z")

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Hi Pavan,

Sorry, I found the issue, removed a partially deleted file from cutadapt step, it worked. Thank you.  
Happy New Year Galaxy folks.

Regards,  
PC