# How to calculate the differential expression without replicate data

**URL:** <https://help.galaxyproject.org/t/how-to-calculate-the-differential-expression-without-replicate-data/7022>\
**Category:** usegalaxy.be support\
**Tags:** limma\_voom\
**Created:** [November 11, 2021, 5:31am UTC](https://help.galaxyproject.org/t/how-to-calculate-the-differential-expression-without-replicate-data/7022 "2021-11-11T05:31:35Z")\
**Posts on this page:** 1\
**Showing post:** 2

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**Author:** ![gallardoalba](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/gallardoalba/32/1903_2.png) [@gallardoalba](https://help.galaxyproject.org/u/gallardoalba)\
**Post date:** [November 17, 2021, 5:16pm UTC](https://help.galaxyproject.org/t/how-to-calculate-the-differential-expression-without-replicate-data/7022/2 "2021-11-17T17:16:51Z")

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Hi @jvd.choudhary,

sorry, but Galaxy restricts edgeR to at least two replicates. You can find more information about this topic in the following post: [DESeq2 or EdgeR or Limma without replication - #7 by jennaj](https://help.galaxyproject.org/t/deseq2-or-edger-or-limma-without-replication/2802/7)

Regards

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