How to combine/merge fastq files? -- Answer/tool: Concatenate datasets tail-to-head (cat)

Dear all,

I am a very new galaxy user. I have 4 fastq files from the same organism, 2 forward reads and 2 reverse reads. I want to merge forward by forward and reverse by reverse before mapping the genome.
I’ve found several tutorials on mapping, which are great, but I don’t know how to merge my sequences.

Could you please kindly help me with this?

1 Like

Hi @HamidGaikani,
you can use the Concatenate datasets tool.

1 Like

Thanks Cristóbal
Much appreciated!