# how to do RNA STAR with Borrelia burgdorferi data **URL:** \ **Category:** usegalaxy.eu support\ **Tags:** custom-genome, reference-genome\ **Created:** [November 6, 2022, 4:00am UTC](https://help.galaxyproject.org/t/how-to-do-rna-star-with-borrelia-burgdorferi-data/8964 "2022-11-06T04:00:48Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![Rooster\_Cui](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/rooster_cui/32/3792_2.png) [@Rooster\_Cui](https://help.galaxyproject.org/u/Rooster_Cui)\ **Post date:** [November 6, 2022, 4:00am UTC](https://help.galaxyproject.org/t/how-to-do-rna-star-with-borrelia-burgdorferi-data/8964/1 "2022-11-06T04:00:48Z")
I am analyzing my RNA-seq data of Borrelia burgdorferi samples with Galaxy, but when I use RNA Star to map the read to the genome, there is no built-in genome to use, I have downloaded one (.gtf) from NCBI, and uploaded in Galaxy, but there is always some issue, anyone know how to do it, thank you very much! ---
**Author:** ![marten](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/marten/32/6_2.png) [@marten](https://help.galaxyproject.org/u/marten)\ **Post date:** [November 7, 2022, 6:49pm UTC](https://help.galaxyproject.org/t/how-to-do-rna-star-with-borrelia-burgdorferi-data/8964/2 "2022-11-07T18:49:43Z")
Have you checked [Custom Genomes - Galaxy Community Hub](https://galaxyproject.org/learn/custom-genomes/) ? RNA Star works with custom genomes but you need to have them in the `fasta` format. ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [December 21, 2023, 12:36am UTC](https://help.galaxyproject.org/t/how-to-do-rna-star-with-borrelia-burgdorferi-data/8964/3 "2023-12-21T00:36:59Z")
Closed on December 21, 2023, 12:36am UTC