# How to make fasta file in a single sequence read like a reference genome?

**URL:** <https://help.galaxyproject.org/t/how-to-make-fasta-file-in-a-single-sequence-read-like-a-reference-genome/13208>\
**Category:** Uncategorized\
**Tags:** assembly\
**Created:** [August 13, 2024, 5:40am UTC](https://help.galaxyproject.org/t/how-to-make-fasta-file-in-a-single-sequence-read-like-a-reference-genome/13208 "2024-08-13T05:40:33Z")\
**Posts on this page:** 3\
**Page:** 1

<div class="post-metadata">

**Author:** ![Matee\_Ullah](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/matee_ullah/32/5933_2.png) [@Matee\_Ullah](https://help.galaxyproject.org/u/Matee_Ullah)\
**Post date:** [August 13, 2024, 5:40am UTC](https://help.galaxyproject.org/t/how-to-make-fasta-file-in-a-single-sequence-read-like-a-reference-genome/13208/1 "2024-08-13T05:40:33Z")

</div>

Hello  
I hope all are doing well.  
I have a query about how to make fasta file into a single read. In the below pic you can see the file is fasta but the sequence read is separated . I want to make them like a reference genome sequence read like in pic 1.  
Pic 2 this is my sequence in fasta format but separated.

 ![17235272840436669469554863038408](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/f/f35a03344d02bd66ea07804b94de0d1e26a0d504.jpeg)  
 ![17235275514488873221034790623839](https://us1.discourse-cdn.com/flex020/uploads/galaxy/original/2X/b/be1771733bc3c3e564f63fce9e7e221b0b0828c3.jpeg)

---

<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [August 13, 2024, 5:59pm UTC](https://help.galaxyproject.org/t/how-to-make-fasta-file-in-a-single-sequence-read-like-a-reference-genome/13208/2 "2024-08-13T17:59:28Z")

</div>

Welcome, @Matee_Ullah

It sounds like you want to _assemble_ your reads.

We have tutorials for **Genomic Assembly** methods in our tutorials here.

> **[Assembly / Tutorial List](https://training.galaxyproject.org/training-material/topics/assembly/)**
>
> DNA sequence data has become an indispensable tool for Molecular Biology & Evolutionary Biology. Study in these fields now require a genome sequence to work from. We call this a 'Reference Sequence.' We need to build a reference for each species....

Please give those a review! 🙂

---

<div class="post-metadata">

**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [April 18, 2026, 1:45am UTC](https://help.galaxyproject.org/t/how-to-make-fasta-file-in-a-single-sequence-read-like-a-reference-genome/13208/3 "2026-04-18T01:45:41Z")

</div>