# How to normalised ChIP-Seq data set with Spike-in

**URL:** https://help.galaxyproject.org/t/how-to-normalised-chip-seq-data-set-with-spike-in/12622
**Category:** Uncategorized
**Tags:** chip-seq, epigenetics
**Created:** [June 3, 2024, 12:34pm UTC](https://help.galaxyproject.org/t/how-to-normalised-chip-seq-data-set-with-spike-in/12622 "2024-06-03T12:34:46Z")
**Posts on this page:** 2
**Page:** 1

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### Author: ![moonmoondeb](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/moonmoondeb/32/5473_2.png) [@moonmoondeb](https://help.galaxyproject.org/u/moonmoondeb)
#### Post date: [June 3, 2024, 12:34pm UTC](https://help.galaxyproject.org/t/how-to-normalised-chip-seq-data-set-with-spike-in/12622/1 "2024-06-03T12:34:46Z")

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Hi  
I am using ChIP-dataset with drosophila spike-in. But can not find out which tool I should use for the spike in normalization in Galaxy.  
Can you kindly help me.  
With regards  
Moonmoon

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### Author: ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)
#### Post date: [June 8, 2024, 1:05am UTC](https://help.galaxyproject.org/t/how-to-normalised-chip-seq-data-set-with-spike-in/12622/2 "2024-06-08T01:05:52Z")

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Hello @moonmoondeb

Several of these tutorials include discussion about how to process and interpret normal versus treated samples → [Epigenetics / Tutorial List](https://training.galaxyproject.org/training-material/topics/epigenetics/)

Other than that, maybe find a few publications that process the data they way you also want to, then use the same or similar processes in Galaxy? If you can share the tool, process, and publication that will make it easier for people at this forum to suggest analogous methods. 🙂