# How to use Seurat in Galaxy? **URL:** \ **Category:** Uncategorized\ **Tags:** gtn-tutorial, transcriptomics, single-cell, deseq2\ **Created:** [May 17, 2023, 3:52pm UTC](https://help.galaxyproject.org/t/how-to-use-seurat-in-galaxy/10077 "2023-05-17T15:52:49Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![liangy10](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/liangy10/32/4290_2.png) [@liangy10](https://help.galaxyproject.org/u/liangy10)\ **Post date:** [May 17, 2023, 3:52pm UTC](https://help.galaxyproject.org/t/how-to-use-seurat-in-galaxy/10077/1 "2023-05-17T15:52:49Z")
Hi I have processed data till gene counts were generated. Since my data are single cells without treatment, I cannot use DESeq2. Is there other ways to visualize the data and group the cells based on their gene expression? I tried Seurat, but got error. Is there a specific format that is required for using Seurat in galaxy? Appreciate if anyone could provide some advice. Kevin ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [May 17, 2023, 5:22pm UTC](https://help.galaxyproject.org/t/how-to-use-seurat-in-galaxy/10077/2 "2023-05-17T17:22:54Z")
Hi @liangy10 Usage is Galaxy is generally the same as the command-line version of tools for file _formats_. The Help section on tool forms try to clarify anything “Galaxy specific” eg what datatype to give the inputs. These tutorials have example data (initial + computed) along with methods (hands-on + workflows). Navigate by analysis domain or search with keywords like tool names. > **[Galaxy Training: Single Cell](https://training.galaxyproject.org/training-material/topics/single-cell/)** > > Training material and practicals for all kinds of single ... The GTN is hosting a training event all next week. GTN materials will be included with live support from scientists from around the globe who work with Galaxy. 🙂 🛠 🧑‍🔬 [Smörgåsbord 3: A week of Free, Online, Galaxy Training supported by the Global Galaxy Training Community](https://help.galaxyproject.org/t/smorgasbord-3-a-week-of-free-online-galaxy-training-supported-by-the-global-galaxy-training-community/9786) ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [February 14, 2025, 9:16pm UTC](https://help.galaxyproject.org/t/how-to-use-seurat-in-galaxy/10077/3 "2025-02-14T21:16:34Z")
Closed on February 14, 2025, 9:16pm UTC