# Identification of novel and known miRNAas using mirdeep2 in galaxy

**URL:** <https://help.galaxyproject.org/t/identification-of-novel-and-known-mirnaas-using-mirdeep2-in-galaxy/11030>\
**Category:** Uncategorized\
**Tags:** mirna, transcriptomics\
**Created:** [October 22, 2023, 11:09am UTC](https://help.galaxyproject.org/t/identification-of-novel-and-known-mirnaas-using-mirdeep2-in-galaxy/11030 "2023-10-22T11:09:23Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Alok\_Dubey](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/alok_dubey/32/4814_2.png) [@Alok\_Dubey](https://help.galaxyproject.org/u/Alok_Dubey)\
**Post date:** [October 22, 2023, 11:09am UTC](https://help.galaxyproject.org/t/identification-of-novel-and-known-mirnaas-using-mirdeep2-in-galaxy/11030/1 "2023-10-22T11:09:23Z")

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Hello everyone,  
Im cureeently working on miRNA data and i need to know the known and novel mirnas present in my samples. im using mirdeep2 for mapping and quantification. Here mirdeep2 is asking input files colapse and maaped files which i have provided it which i got via mirddep2 mapper and genome file which i have downloaded from ncbi, after providing everything im getting error. Please, can anyone gere guide me through it.

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [October 23, 2023, 10:33pm UTC](https://help.galaxyproject.org/t/identification-of-novel-and-known-mirnaas-using-mirdeep2-in-galaxy/11030/2 "2023-10-23T22:33:30Z")

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Hi @Alok_Dubey

This tutorial has very good example data, and a workflow template that you could adopt: [Whole transcriptome analysis of Arabidopsis thaliana](https://training.galaxyproject.org/training-material/topics/transcriptomics/tutorials/mirna-target-finder/tutorial.html)

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [February 14, 2025, 9:07pm UTC](https://help.galaxyproject.org/t/identification-of-novel-and-known-mirnaas-using-mirdeep2-in-galaxy/11030/3 "2025-02-14T21:07:00Z")

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