# I'm encountering errors while running hicPCA analysis and need assistance **URL:** \ **Category:** Uncategorized\ **Tags:** troubleshooting\ **Created:** [July 14, 2026, 3:22pm UTC](https://help.galaxyproject.org/t/im-encountering-errors-while-running-hicpca-analysis-and-need-assistance/18166 "2026-07-14T15:22:31Z")\ **Posts on this page:** 1\ **Showing post:** 2
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [July 14, 2026, 3:31pm UTC](https://help.galaxyproject.org/t/im-encountering-errors-while-running-hicpca-analysis-and-need-assistance/18166/2 "2026-07-14T15:31:26Z")
Welcome @ahl Hopefully we can help! The tool seems to be reporting an issue with one of the input file’s genome coordinates. This could be in the BED file or one of the other datasets input to the tool. In short, a coordinate range is probably either unreadable for some reason (extra headers?) or the data might be based on different genome assemblies (this too is somewhat common) and falling outside of the coordinate range it is being compared to. I would suggest reviewing formats first, then the data provider where you sourced the data to examine the assembly version of each. If you used a genome assembly in Galaxy for some step, most of those were sourced from UCSC, but not all, and the full description has some details. Please ask if anything is not clear! This guide is about human but the advice can apply to any genome with multiple assembly choices. - [Reference genomes at public Galaxy servers: GRCh38/hg38 example](https://help.galaxyproject.org/t/reference-genomes-at-public-galaxy-servers-grch38-hg38-example/11616) Does this help you to know what to review in the run? If you get stuck, we can help more here if you share your history for some feedback, since the details will matter and screenshots will likely not be enough. Once we are done, you can unshare. - [FAQ: Sharing your History](https://galaxyproject.github.io/training-material/faqs/galaxy/histories_sharing.html) Let’s start there! 🙂 --- _[View the full topic](https://help.galaxyproject.org/t/im-encountering-errors-while-running-hicpca-analysis-and-need-assistance/18166)._