Hi! I’m trying to align fastq files to the built in mouse genome (GRCm39/mm39) using RNA STAR. I am getting errors of “Encountered an unhandled exception while caching job destination dynamic rule” almost immediately or near immediately after submitting the job. I’ve tried with different fastq files. These fastq files work successfully when using HISAT2.
I cannot reproduce the issue with RNA_STAR on the ORG server. SE reads (a tiny file with 1k reads), mm39 built-in genome without annotation, no gff file, individual jobs (not a collection). I tried mm10, but that job is still running. The run time was rather excessive considering tiny tests datasets.
We had a small configuration issue at UseGalaxy.org last Friday. This is now resolved and a rerun should work now! Please give this a try and let us know.