# LEfSe comparing more than two classes **URL:** \ **Category:** usegalaxy.org support\ **Tags:** troubleshooting, lefse\ **Created:** [July 7, 2023, 2:49pm UTC](https://help.galaxyproject.org/t/lefse-comparing-more-than-two-classes/10418 "2023-07-07T14:49:57Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![microbiome](https://avatars.discourse-cdn.com/v4/letter/m/5f9b8f/32.png) [@microbiome](https://help.galaxyproject.org/u/microbiome)\ **Post date:** [July 7, 2023, 2:49pm UTC](https://help.galaxyproject.org/t/lefse-comparing-more-than-two-classes/10418/1 "2023-07-07T14:49:57Z")
Hi everyone, This is a question for the LEfSe galaxy module: I have a question about comparing more than two classes. Is this possible in the module? I tried this but the results were a bit unexpected. When I compare, for example, class A and class B or A and C or A and B, I get several distinct differences. However, when I compare A, B, and C together, I get only one difference that never appeared in the individual comparisons? Is that an expected result? Would you have any advice on this? Thank you! ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [July 7, 2023, 5:04pm UTC](https://help.galaxyproject.org/t/lefse-comparing-more-than-two-classes/10418/2 "2023-07-07T17:04:27Z")
Hi @microbiome There are a few versions of Lefse in the Galaxy community. I _think_ Lefse only produces a single comparison per run for the versions hosted at ORG, EU, and AU. Not sure about Biobakery’s version. Maybe that explains your observations? ---
**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\ **Post date:** [January 16, 2025, 6:09pm UTC](https://help.galaxyproject.org/t/lefse-comparing-more-than-two-classes/10418/3 "2025-01-16T18:09:40Z")
Closed on January 16, 2025, 6:09pm UTC