# Length of Quality Sequence and Length of read do not match

**URL:** <https://help.galaxyproject.org/t/length-of-quality-sequence-and-length-of-read-do-not-match/4290>\
**Category:** Resources\
**Tags:** resources, quality-control, iwc-workflows\
**Created:** [August 14, 2020, 4:59am UTC](https://help.galaxyproject.org/t/length-of-quality-sequence-and-length-of-read-do-not-match/4290 "2020-08-14T04:59:02Z")\
**Posts on this page:** 1\
**Showing post:** 2

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**Author:** ![jennaj](https://sea2.discourse-cdn.com/flex020/user_avatar/help.galaxyproject.org/jennaj/32/27_2.png) [@jennaj](https://help.galaxyproject.org/u/jennaj)\
**Post date:** [April 18, 2025, 10:02pm UTC](https://help.galaxyproject.org/t/length-of-quality-sequence-and-length-of-read-do-not-match/4290/2 "2025-04-18T22:02:39Z")

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For anyone reading this later on:

Check your job details page [using the i-icon](https://training.galaxyproject.org/training-material/faqs/galaxy/datasets_icons.html). Do you see this within the message? Or a similar message about the fastq format? If yes, then your fastq data is not intact for some reason.

> Sequence and quality length don’t match

**Common reasons:**

1. Manipulations done somewhere else impacted the file content
2. Or, a truncated file was created when moving the data between storage locations, including a potentially interrupted Upload job to Galaxy

### 🚀 Galaxy resources!

- To learn about NGS reads and how to inspect them, please start here: [Hands-on: NGS data logistics / NGS data logistics / Introduction to Galaxy Analyses](https://training.galaxyproject.org/training-material/topics/introduction/tutorials/galaxy-intro-ngs-data-managment/tutorial.html)

- To learn how to Upload data to Galaxy, please see: [Getting Data into Galaxy](https://help.galaxyproject.org/t/getting-data-into-galaxy/10868).

- To double check for intact files and the current state of compression, try the tool **Fastq info** at any UseGalaxy server.

- To check the quality content of fastq data, try the tool **FastQC**.

- To see what a simple Quality Control workflow looks like, please see → [What do these FastQC results mean? - #4 by jennaj](https://help.galaxyproject.org/t/what-do-these-fastqc-results-mean/15121/4)

- More workflows can be found at:

🎓 New to workflows? Start here: [Hands-on: Galaxy Basics for everyone / Galaxy Basics for everyone / Introduction to Galaxy Analyses](https://training.galaxyproject.org/training-material/topics/introduction/tutorials/galaxy-intro-101-everyone/tutorial.html)

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